PMID: 9180164May 29, 1997Paper

Isolation from a human MDR lung cell line of multiple clonal subpopulations which exhibit significantly different drug resistance

International Journal of Cancer. Journal International Du Cancer
M HeenanM Clynes

Abstract

The heterogeneous nature of an adriamycin-selected human MDR squamous lung cell line, DLKP-A, was investigated by isolating and characterising 9 of its clonal subpopulations. The DLKP-A cell line exhibits resistance to the classical MDR drugs, overexpresses P-glycoprotein and displays reduced topoisomerase II amounts. The clonal cell lines exhibit a wide range of resistance extents, with the most resistant clone displaying 9 times the extent of adriamycin resistance observed in the least resistant clone. A number of clones exhibit sensitivity to the concentration of adriamycin in which the parental cell line was selected, possibly indicating cooperation between the more and less resistant cells. Detailed analysis of 4 of the clonal subpopulations revealed broadly similar drug resistance mechanisms. Alterations in expression of the MDR-associated genes MDR1 and Topo IIalpha were observed, with no detectable changes in the expression of MDR3, MRP, GSTpi, Topo IIbeta, Topo I and CYP1A1 noted. However, each clonal cell line displayed a distinct extent of expression of MDR1 and Topo IIalpha and further characterisation of the clones indicated that other modes of drug resistance may exist in at least one of the cell lines. In particu...Continue Reading

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Citations

Apr 4, 2015·Expert Opinion on Drug Metabolism & Toxicology·Helena JoyceAnnemarie Larkin
Nov 22, 2007·Expert Opinion on Drug Metabolism & Toxicology·Robert O'ConnorLorraine O'Driscoll
Oct 25, 2016·Cancer Letters·Yiwen BuDeliang Cao
Oct 15, 2009·Journal of Experimental & Clinical Cancer Research : CR·Naomi WalshNorma O'Donovan
Feb 6, 1999·International Journal of Cancer. Journal International Du Cancer·A LarkinM Clynes
Jun 1, 2010·Journal of Neuro-oncology·Paula KinsellaVerena Amberger-Murphy
Jan 14, 2020·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·A ToliosT Mohr
Jan 26, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Charles O'DohertyRichard Murphy
May 4, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y LiangM Clynes
Jun 16, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C O'LoughlinM Clynes

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