Jan 1, 1976

Isolation from bovine brain of a fraction containing capillaries and a fraction containing membrane fragments of the choroid plexus

Journal of Neurobiology
G SessaJ P Green


Combined differential and density gradient centrifugation was used for the isolation of a capillary-rich fraction from the cerebral cortex and a brush border containing fraction from the bovine choroid plexus. The activities of gamma-glutamyl transpeptidase and several other marker enzymes were monitored during the fractionation procedure. Electron microscopic examination showed a membrane-rich fraction in the choroid plexus high in the gamma-glutamyl transpeptidase and 5'-nucleotidase activities. From the brain cortex, a capillary-rich fraction was obtained which was high in gamma-glutamyl transpeptidase and alkaline phosphatase activities. A histochemical examination showed gamma-glutamyl transpeptidase activity localized in the capillary walls.

Mentioned in this Paper

Centrifugation, Density Gradient
Acid Phosphatase
Tissue Membrane
Gamma-glutamyl transferase
Alkaline Phosphatase
Insula of Reil
Bos indicus
Cell Separation

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