Isolation from cochlea of a novel human intronless gene with predominant fetal expression

Journal of the Association for Research in Otolaryngology : JARO
Barbara L ResendesCynthia C Morton

Abstract

We have cloned a novel human gene, designated PFET1 (predominantly fetal expressed T1 domain) (HUGO-approved symbol KCTD12 or C13orf2), by subtractive hybridization and differential screening of human fetal cochlear cDNA clones. Also, we have identified the mouse homolog, designated Pfet1. PFET1/Pfet1 encode a single transcript of approximately 6 kb in human, and three transcripts of approximately 4, 4.5, and 6 kb in mouse with a 70% GC-rich open reading frame (ORF) consisting of 978 bp in human and 984 bp in mouse. Both genes have unusually long 3' untranslated (3' UTR) regions (4996 bp in human PFET1, 3700 bp in mouse Pfet1) containing 12 and 5 putative polyadenylation consensus sequences, respectively. Pfetin, the protein encoded by PFET1/Pfet1, is predicted to have 325 amino acids in human and 327 amino acids in mouse and to contain a voltage-gated potassium (K+) channel tetramerization (T1) domain. Otherwise, to date these genes have no significant homology to any known gene. PFET1 maps to the long arm of human chromosome 13, in band q21 as shown by FISH analysis and STS mapping. Pfet1 maps to mouse chromosome 14 near the markers D14Mit8, D14Mit93, and D14Mit145.1. The human 6 kb transcript is present in a variety of fetal...Continue Reading

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Citations

Mar 9, 2011·International Journal of Clinical Oncology·Yoshiyuki Suehara
May 24, 2006·Journal of the Association for Research in Otolaryngology : JARO·Theru A SivakumaranCynthia C Morton
Jun 2, 2007·Journal of the Association for Research in Otolaryngology : JARO·Irene S GabashviliAnne B S Giersch
Feb 22, 2012·Development, Growth & Differentiation·Naouel GharbiAnders Fjose
Jul 11, 2012·International Journal of Proteomics·Tadashi KondoAkira Kawai
Apr 14, 2009·Journal of Molecular Biology·Irina S DementievaSteve A N Goldstein
Feb 16, 2007·Hearing Research·Michael S HildebrandHans-Henrik M Dahl
Feb 13, 2007·Brain Research·Sonia M S Rocha-SanchezKirk W Beisel
Jun 25, 2008·The FEBS Journal·Yolanda BayónAndrés Alonso
Apr 1, 2011·The Journal of Comparative Neurology·Michaela MetzBernhard Bettler
Feb 6, 2016·Scientific Reports·Liping LiTiebang Kang
Sep 10, 2011·Japanese Journal of Clinical Oncology·Daisuke KubotaTadashi Kondo
Oct 19, 2017·Expert Review of Proteomics·Tadashi Kondo
Apr 14, 2010·Molecular Psychiatry·M T M LeeA T A Cheng
Apr 25, 2007·Annals of Neurology·Patrick Van BogaertMarc J Abramowicz
Jun 7, 2008·Clinical Orthopaedics and Related Research·Akira KawaiSetsuo Hirohashi
May 19, 2012·Nature Reviews. Neuroscience·Martin Gassmann, Bernhard Bettler
Mar 25, 2020·Journal of Clinical Laboratory Analysis·Run-Yi YeShen-Ming Wang
Apr 22, 2010·The Journal of Biological Chemistry·Tudor BartoiHans-Christian Kornau
Aug 31, 2018·BMC Cancer·Mohammad Reza AbbaszadeganMeysam Moghbeli
May 19, 2021·Cell Communication and Signaling : CCS·Annapaola AngrisaniMarta Moretti

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