PMID: 3214164Dec 1, 1988Paper

Isolation from earthworms of a proteinaceous chemoattractant to garter snakes

Archives of Biochemistry and Biophysics
D WangM Halpern

Abstract

We have isolated a potent proteinaceous chemoattractant from aqueous washes of earthworm (Lumbricus terrestris) for garter snakes (Thamnophis sirtalis) by means of a covalent chromatography. It contained free sulfhydryl groups and showed an apparent mass of 20 kDa. The chemoattractive activity of this protein could be destroyed by heating as well as by proteolysis. Its activity could also be reversibly blocked by mixed disulfide formation with dithiodipyridine, suggesting that the free sulfhydryl(s) was essential for its function as a chemoattractant. This bioactive material had a tendency to form intermolecular crosslinked aggregates during isolation, if reducing agents were not included. Some of the high-molecular-weight aggregates cochromatographed with earthworm cuticle collagen on Ultragel AcA 34 or 44 columns. In contrast to an earlier report by D. M. Kirschenbaum, N. Schulman, and M. Halpern [1986) Proc. Natl. Acad. Sci. USA 83, 1213-1216) the purified earthworm collagen showed no chemoattractive activity to garter snakes.

References

Feb 1, 1975·Physiology & Behavior·G M Burghardt, C H Pruitt
Sep 1, 1975·Journal of Comparative and Physiological Psychology·J Kubie, M Halpern
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D M KirschenbaumM Halpern
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D M KirschenbaumM Halpern
Oct 1, 1968·Journal of Comparative and Physiological Psychology·G M Burghardt, E H Hess
Mar 1, 1967·Archives of Biochemistry and Biophysics·D R Grassetti, J F Murray
Oct 1, 1984·Pharmacology, Biochemistry, and Behavior·M HalpernD M Kirschenbaum
Mar 1, 1958·The Biochemical Journal·M R WATSON

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Citations

Oct 1, 1989·Pharmacology, Biochemistry, and Behavior·X C JiangM Halpern
Apr 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D WangM Halpern
Dec 5, 2002·Archives of Biochemistry and Biophysics·Dalton WangMimi Halpern
Sep 3, 2003·Progress in Neurobiology·Mimi Halpern, Alino Martínez-Marcos
Aug 11, 2006·Molecular & Cellular Proteomics : MCP·Baptiste LeroyRuddy Wattiez
Mar 6, 2002·Journal of Neurophysiology·Angel R CinelliMimi Halpern

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