PMID: 7537972Feb 1, 1995Paper

Isolation from human brain of six previously unreported cDNAs related to the reverse transcriptase of human endogenous retroviruses

AIDS Research and Human Retroviruses
S LefebvreJ F Bureau

Abstract

cDNAs prepared from total RNA extracted from plaques of multiple sclerosis were amplified by the polymerase chain reaction. The 11-bp degenerate primers used were derived from conserved sequences of reverse transcriptase. Amplified cDNAs were fractionated according to size by electrophoresis in polyacrylamide gels under denaturing conditions. cDNAs of the proper size were cloned, grouped according to the sequence of their insert by differential hybridization, and sequenced. Six cDNAs were isolated and found to belong to new members of two groups of human endogenous retroviruses: the group related to ERV9 and that related to HERVK10 and HUMMTV. These sequences were expressed in all human organs tested, including normal white matter of brain. The approach described in this article is a powerful tool with which to isolate new members of the reverse transcriptase gene family.

References

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Citations

Dec 24, 1997·Annals of Neurology·M Brahic, J F Bureau
Nov 11, 1999·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·P Monteyne
Apr 30, 1999·AIDS Research and Human Retroviruses·P OlssonJ Blomberg
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Cécile VoissetDavid J Griffiths
Oct 3, 1999·The Journal of Investigative Dermatology·C HohenadlC Leib-Mösch
Mar 19, 2014·Neuropathology and Applied Neurobiology·Rolf BusleiReiner Strick
Sep 2, 1998·Current Opinion in Neurology·P MonteyneM Brahic
Jun 1, 1995·Multiple Sclerosis : Clinical and Laboratory Research·P Froussard

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