PMID: 10074108Mar 12, 1999Paper

Isolation from tobacco mosaic virus-infected tobacco of a solubilized template-specific RNA-dependent RNA polymerase containing a 126K/183K protein heterodimer

Journal of Virology
T WatanabeA Ishihama

Abstract

The complete nucleotide sequence was determined for the putative RNA polymerase (183K protein) gene of tobacco mosaic virus (TMV) OM strain, which differed from the related strain, vulgare, by 51 positions in its nucleotide sequence and 6 residues in its amino acid sequence. Three segments of this 183K protein, each containing the sequence motif of methyltransferase (M), helicase (H), or RNA-dependent RNA polymerase (P), were expressed in Escherichia coli as fusion proteins with hexahistidine tags, and domain-specific antibodies were raised against purified His-tagged M and P polypeptides. By immunoaffinity purification, a template-specific RNA-dependent RNA polymerase containing a heterodimer of the full-length 183K and 126K (an amino-terminal-proximal portion of the 183K protein) viral proteins was isolated. We propose that the TMV RNA polymerase for minus-strand RNA synthesis is composed of one molecule each of the 183- and 126-kDa proteins, possibly together with two or more host proteins.

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Citations

Apr 9, 2008·Molecular Plant-microbe Interactions : MPMI·Atsushi YoshiiTadaaki Hibi
Jan 1, 2014·Uirusu·Kazuhiro Ishibashi, Masayuki Ishikawa
Nov 16, 2017·Molecular Plant-microbe Interactions : MPMI·Nachelli Malpica-LópezMikhail M Pooggin
May 29, 2004·The Journal of General Virology·Hai-He Wang, Sek-Man Wong
Jun 23, 2007·The Plant Journal : for Cell and Molecular Biology·Vitaly BoykoManfred Heinlein
Sep 28, 2004·The Journal of General Virology·Tomas CantoPeter Palukaitis
May 1, 2001·Molecular Plant Pathology·E Knapp, D J Lewandowski
Feb 6, 2013·The Plant Journal : for Cell and Molecular Biology·Annette NiehlManfred Heinlein
Mar 3, 2018·Nature Plants·Camilla Julie KørnerManfred Heinlein

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