Isolation, genome sequencing and functional analysis of two T7-like coliphages of avian pathogenic Escherichia coli

Gene
Mianmian ChenWei Zhang

Abstract

Avian pathogenic Escherichia coli (APEC) causes colibacillosis, which results in significant economic losses to the poultry industry worldwide. Due to the drug residues and increased antibiotic resistance caused by antibiotic use, bacteriophages and other alternative therapeutic agents are expected to control APEC infection in poultry. Two APEC phages, named P483 and P694, were isolated from the feces from the farmers market in China. We then studied their biological properties, and carried out high-throughput genome sequencing and homology analyses of these phages. Assembly results of high-throughput sequencing showed that the structures of both P483 and P694 genomes consist of linear and double-stranded DNA. Results of the electron microscopy and homology analysis revealed that both P483 and P694 belong to T7-like virus which is a member of the Podoviridae family of the Caudovirales order. Comparative genomic analysis showed that most of the predicted proteins of these two phages showed strongest sequence similarity to the Enterobacteria phages BA14 and 285P, Erwinia phage FE44, and Kluyvera phage Kvp1; however, some proteins such as gp0.6a, gp1.7 and gp17 showed lower similarity (<85%) with the homologs of other phages in th...Continue Reading

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Citations

Sep 25, 2017·Applied and Environmental Microbiology·Mianmian ChenWei Zhang
Nov 15, 2018·Viruses·Alexander B CarstensLars H Hansen
Nov 30, 2018·PloS One·Temitope Cyrus Ekundayo, Anthony I Okoh
Sep 18, 2020·Scientific Reports·Patricia E SørensenPatrick Butaye
Jul 9, 2020·Frontiers in Microbiology·Karolina FilikEwa Brzozowska

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