Isolation, identification of sludge-lysing strain and its utilization in thermophilic aerobic digestion for waste activated sludge

Bioresource Technology
Xuesong LiHiroaki I Ogawa

Abstract

A strain of sludge-lysing bacteria was isolated from waste activated sludge (WAS) in this study. The result of 16S rRNA gene analysis demonstrated that it was a species of new genus Brevibacillus (named Brevibacillus sp. KH3). The strain could release the protease with molecule weight of about 40 kDa which could enhance the efficiency of sludge thermophilic aerobic digestion. During the sterilized sludge digestion experiment inoculated with Brevibacillus sp. KH3, the maximum protease activity was 0.41 U/ml at pH 8 and 50 degrees C, and maximum TSS removal ratio achieved 32.8% after 120 h digestion at pH 8 and 50 degrees C. In the case of un-sterilized sludge digestion inoculated with Brevibacillus sp. KH3, TSS removal ratio in inoculated-group was 54.8%, increasing at 11.86% compared with un-inoculation (46.2%). The result demonstrated that inoculation of Brevibacillus sp. KH3 could help to degrade the EPS and promote the collapse of cells and inhibit the growth of certain kinds of microorganisms. It indicated that Brevibacillus sp. KH3 strain had a high potential to enhance WAS-degradation efficiency in thermophilic aerobic digestion.

References

Jan 1, 1991·Journal of Bacteriology·W G WeisburgD J Lane
May 10, 1995·Archives of Biochemistry and Biophysics·K J RaserK K Wang
May 15, 2002·Journal of Biotechnology·Hong Liu, Herbert H P Fang
Jun 21, 2003·Science·José E González-PastorRichard Losick
Nov 30, 2005·Bioresource Technology·Ruth Gebauer, Bjørnar Eikebrokk
Feb 17, 2006·Bioresource Technology·P H L NguyenC Visvanathan
Jul 13, 2006·Bioresource Technology·Sebastian Borowski, Józef Stanisław Szopa
Feb 9, 2007·Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology·Yoshiyuki OkumuraToshiaki Nikai

❮ Previous
Next ❯

Citations

Apr 10, 2013·Bioresource Technology·Mohd Zulkhairi Mohd YusoffChang-Ping Yu
Jul 23, 2013·Environmental Science and Pollution Research International·J MerrylinJ Rajesh Banu
May 18, 2013·Applied Microbiology and Biotechnology·Fan LüPinjing He
May 6, 2014·Journal of Industrial Microbiology & Biotechnology·Minh Tuan NguyenHiroaki I Ogawa
Mar 17, 2009·Journal of Hazardous Materials·Toshinari MaedaHiroaki I Ogawa
Jun 21, 2013·Journal of Applied Microbiology·K FujiiA Mikami
Nov 28, 2013·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·In-Soo KimSung-Cheol Koh
Apr 28, 2016·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Liang GuoZonglian She
Apr 15, 2010·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Chul-Hwi ParkGee-Bong Han
Dec 12, 2020·Bioresource Technology·Qili ZhuToshinari Maeda
Jul 3, 2021·International Journal of Environmental Research and Public Health·Hongyan MuQingzhe Jiang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.