Isolation, immortalization, and characterization of a human breast epithelial cell line with stem cell properties

Genes & Development
Thorarinn GudjonssonOle William Petersen

Abstract

The epithelial compartment of the human breast comprises two distinct lineages: the luminal epithelial and the myoepithelial lineage. We have shown previously that a subset of the luminal epithelial cells could convert to myoepithelial cells in culture signifying the possible existence of a progenitor cell. We therefore set out to identify and isolate the putative precursor in the luminal epithelial compartment. Using cell surface markers and immunomagnetic sorting, we isolated two luminal epithelial cell populations from primary cultures of reduction mammoplasties. The major population coexpresses sialomucin (MUC(+)) and epithelial-specific antigen (ESA(+)) whereas the minor population has a suprabasal position and expresses epithelial specific antigen but no sialomucin (MUC(-)/ESA(+)). Two cell lines were further established by transduction of the E6/E7 genes from human papilloma virus type 16. Both cell lines maintained a luminal epithelial phenotype as evidenced by expression of the tight junction proteins, claudin-1 and occludin, and by generation of a high transepithelial electrical resistance on semipermeable filters. Whereas in clonal cultures, the MUC(+)/ESA(+) epithelial cell line was luminal epithelial restricted in ...Continue Reading

References

Feb 1, 1992·International Journal of Cancer. Journal International Du Cancer·T M PaineP J Dawson
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·O W PetersenM J Bissell
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·V BandR Sager
Nov 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A SapinoG Bussolati
Dec 1, 1988·Differentiation; Research in Biological Diversity·O W Petersen, B van Deurs
Jun 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·O W Petersen, B van Deurs
Jun 1, 1995·Seminars in Cancer Biology·V Band
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·D E WazerV Band
Jan 1, 1994·Methods in Enzymology·R J BlaschkeM J Bissell
Oct 6, 1997·The Journal of Membrane Biology·M M JaegerB Kachar
May 29, 1998·The Journal of Pathology·R AnbazhaganB A Gusterson
Jun 6, 1998·The Journal of Pathology·L Bouwens
Aug 26, 1998·Advances in Cancer Research·O W PetersenM J Bissell
Sep 24, 1998·Differentiation; Research in Biological Diversity·J StinglJ T Emerman
Jan 27, 1999·Developmental Biology·C PéchouxO W Petersen
May 18, 1999·International Journal of Cancer. Journal International Du Cancer·L QueimadoM Lovett
Nov 24, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J SmalleyM J O'Hare
Dec 22, 1999·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·M BalzarS V Litvinov
Aug 19, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·D L HudsonJ R Masters
Nov 4, 2000·The Journal of Cell Biology·Y TamaiM M Taketo
Jan 13, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D L HudsonJ R Masters
Feb 13, 2001·Microscopy Research and Technique·G H Smith, G Chepko
Mar 23, 2001·Breast Cancer Research and Treatment·S J SantnerF R Miller
Aug 25, 2001·Breast Cancer Research and Treatment·J StinglJ T Emerman

❮ Previous
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Citations

Sep 2, 2005·Journal of Cancer Research and Clinical Oncology·Bin-Bin LiuYin-Kun Liu
Apr 19, 2007·Cell and Tissue Research·Mark D CreganPeter E Hartmann
Mar 17, 2006·Breast Cancer Research and Treatment·Kate C ShenY Alan Wang
Mar 17, 2006·Breast Cancer Research and Treatment·Cheryl M LewisDavid M Euhus
Sep 5, 2009·Breast Cancer Research and Treatment·Jessica C LawsonAdrienne L Edkins
Oct 21, 2010·Investigational New Drugs·Linda VidarsdottirJorunn Erla Eyfjord
Apr 20, 2005·Journal of Mammary Gland Biology and Neoplasia·Kenna R Mills ShawJoan S Brugge
May 12, 2005·Journal of Mammary Gland Biology and Neoplasia·John StinglConnie J Eaves
May 12, 2005·Journal of Mammary Gland Biology and Neoplasia·Agla Jael Rubner FridriksdottirOle William Petersen
Jun 30, 2006·Journal of Mammary Gland Biology and Neoplasia·Thorarinn GudjonssonMina J Bissell
Jun 30, 2006·Journal of Mammary Gland Biology and Neoplasia·Catherine ClarkeSunil R Lakhani
Nov 9, 2012·Pharmaceutical Research·Berglind Eva BenediktsdóttirOlafur Baldursson
Sep 19, 2007·In Vitro Cellular & Developmental Biology. Animal·Skarphedinn HalldorssonThorarinn Gudjonsson
Sep 18, 2007·Stem Cell Reviews·François VaillantJane E Visvader
Sep 18, 2007·Stem Cell Reviews·Mark A LaBargeMina J Bissell
Jun 12, 2003·Methods : a Companion to Methods in Enzymology·Thorarinn GudjonssonOle William Petersen
Apr 28, 2004·Breast Cancer Research and Treatment·Jong Bin KimMike J O'Hare
Sep 30, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Kristin C JensenMatt van de Rijn
Dec 11, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Christopher Y ParkIrving L Weissman
Mar 8, 2006·Nature Clinical Practice. Oncology·Sanford H Barsky, Nina J Karlin
Jan 30, 2007·Nature Clinical Practice. Oncology·Klaus Pantel, Catherine Alix-Panabières
Aug 2, 2005·Nature Reviews. Cancer·Britta WeigeltLaura J van 't Veer
Sep 14, 2007·Nature Reviews. Cancer·John Stingl, Carlos Caldas
Jan 10, 2009·Nature Reviews. Cancer·Barry Gusterson
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xiangshan ZhaoVimla Band
Sep 24, 2011·Proceedings of the National Academy of Sciences of the United States of America·Patricia J KellerCharlotte Kuperwasser
Jun 25, 2008·Stem Cells and Development·Julie DouvilleDanuta Balicki
Jan 31, 2013·Stem Cells and Development·Bizunesh M BorenaGerlinde R Van de Walle
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Ole William Petersen, Kornelia Polyak
Jul 9, 2010·Cold Spring Harbor Perspectives in Biology·Tim H-M Huang, Manel Esteller
Oct 12, 2010·Cold Spring Harbor Perspectives in Biology·Jane E Visvader, Gilbert H Smith
May 21, 2003·Genes & Development·Gabriela DontuMax S Wicha
Oct 25, 2006·Molecular and Cellular Biology·Shelley A GestlEdward J Gunther
Jan 20, 2004·Journal of Medical Genetics·W D Foulkes
Jan 1, 2008·Journal of Oncology·Claudia CasarsaDanila Coradini
Nov 4, 2010·Cancer Research·Tao LinRobert Y L Tsai
Nov 3, 2007·The Journal of Clinical Investigation·Kornelia Polyak

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