Isolation of β-carotene, α-carotene and lutein from carrots by countercurrent chromatography with the solvent system modifier benzotrifluoride

Journal of Chromatography. a
Michael EnglertWalter Vetter

Abstract

A carotenoid purification method with dual-mode countercurrent chromatography (CCC) for β-carotene, α-carotene and lutein from a fresh carrot extract was developed. The fluorinated liquid benzotrifluoride (IUPAC name: (trifluoromethyl)benzene) was used as a novel modifier in the non-aqueous ternary solvent system n-hexane/benzotrifluoride/acetonitrile. The ternary phase diagram of the type I solvent system was used to select two-phase solvent mixtures which enabled an efficient preparative separation of α-carotene, β-carotene and lutein from concomitant pigments in crude carrot extract. By means of the modifier, high separation factors (α ≥ 1.2) were obtained, allowing baseline resolution between α-carotene and β-carotene due to specific chemical interactions such as π-π molecular interactions. After optimizing the injection step with a pseudo-ternary phase diagram, 51 mg of β-carotene, 32 mg of α-carotene and 4 mg of lutein could be isolated from 100.2mg crude carrot extract in a short time and with high purities of 95% and 99% by using dual-mode CCC, respectively. Temperatures > 22°C had a negative impact on the separation of α-carotene and β-carotene.

References

Jun 13, 2000·Critical Reviews in Food Science and Nutrition·F Delgado-VargasO Paredes-López
Jul 25, 2000·Journal of Chromatography. a·J Oliver, A Palou
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Qing SuNicholas D H Balazs
Mar 14, 2007·Journal of Chromatography. a·Susanne BaldermannPeter Fleischmann
Nov 26, 2013·Chemical Reviews·Rosana ÁlvarezÁngel R de Lera

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Citations

Aug 27, 2015·Marine Drugs·Montserrat Rodrigo-BañosRosa María Martínez-Espinosa
Dec 21, 2019·Analytical and Bioanalytical Chemistry·Marco MüllerWalter Vetter
Jun 13, 2018·Marine Drugs·Javier Torregrosa-CrespoRosa María Martínez-Espinosa
Apr 30, 2019·Journal of Chromatography. a·Walter VetterSimon Hammann

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