Isolation of a calcium-binding phosphoprotein from the oocytes and hemolymph of the blood-sucking insect Rhodnius prolixus.

The Journal of Biological Chemistry
Mário A C Silva-NetoHatisaburo Masuda

Abstract

A novel calcium-binding phosphoprotein was isolated from the oocytes of the blood-sucking bug Rhodnius prolixus. This protein exhibits an apparent molecular mass of 18 kDa on gel filtration, but migrates as an 8-kDa band on N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine/SDS-polyacrylamide gels. It has a high content of serine (24% of the total number of residues), and phosphoserine is the sole amino acid phosphorylated in vivo. A similar protein was partially purified from the hemolymph. It resembles the oocyte form of the protein in its NH2-terminal sequence and its ability to be taken up by growing ovaries. 45Ca binding to the oocyte phosphoprotein was determined after SDS-polyacrylamide gel electrophoresis followed by blotting on nitrocellulose membranes. Titration of Ca2+-binding sites shows a high capacity (approximately 50 mol/mol of protein), but a low affinity (K0.5 congruent with 10(-3) M). Based on these characteristics, we have named this protein Rhodnius calcium-binding phosphoprotein. It resembles phosvitin, a phosphoprotein present in the oocytes of nonmammalian vertebrates.

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Citations

Jan 1, 1997·Archives of Insect Biochemistry and Physiology·H S CoelhoH Masuda
Jan 9, 1999·Archives of Insect Biochemistry and Physiology·E A MachadoH Masuda
Dec 5, 2000·Insect Biochemistry and Molecular Biology·A Costa-FilhoL F Silva
Mar 14, 2002·Insect Biochemistry and Molecular Biology·Marcelo N MedeirosEdnildo A Machado
Jul 12, 2002·Insect Biochemistry and Molecular Biology·Mário A C Silva-NetoHatisaburo Masuda
Jul 12, 2002·Insect Biochemistry and Molecular Biology·Eliane FialhoMário A C Silva-Neto
Apr 2, 2014·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Leonardo Rodrigues CunhaMário Alberto Cardoso da Silva-Neto
Jun 26, 2015·Archives of Insect Biochemistry and Physiology·Marcia C PaesGeorgia C Atella
Jun 11, 2002·Biochemical and Biophysical Research Communications·Mário A C Silva-NetoAngela H C S Lopes
Mar 1, 2006·Insect Biochemistry and Molecular Biology·Alan B SilveiraMário A C Silva-Neto
Aug 30, 2005·Anais Da Academia Brasileira De Ciências·Georgia C AtellaHatisaburo Masuda
Mar 18, 2004·Archives of Insect Biochemistry and Physiology·M N MedeirosE A Machado
Sep 8, 2004·Archives of Insect Biochemistry and Physiology·Rodrigo B FerraroJosé R Meyer-Fernandes
Jul 27, 2006·Molecular Reproduction and Development·I B RamosE A Machado
Aug 22, 2006·Archives of Insect Biochemistry and Physiology·D M P Oliveira, E A Machado
May 13, 2003·The Journal of Biological Chemistry·Daniel M GolodneGeorgia C Atella
Mar 31, 2005·Archives of Biochemistry and Biophysics·Eliane FialhoMário A C Silva-Neto

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