Isolation of a cDNA encoding a beta-1,4-endoglucanase in the root-knot nematode Meloidogyne incognita and expression analysis during plant parasitism

Molecular Plant-microbe Interactions : MPMI
M N RossoP Abad

Abstract

A beta-1,4-endoglucanase encoding cDNA (EGases, E.C. 3.2.1.4), named Mi-eng-1, was cloned from Meloidogyne incognita second-stage juveniles (J2). The deduced amino acid sequence contains a catalytic domain and a cellulose-binding domain separated by a linker. In M. incognita, the gene is transcribed in the migratory J2, in males, and in the sedentary adult females. In pre-parasitic J2, endoglucanase transcripts are located in the cytoplasm of the subventral esophageal glands. The presence of beta-1,4-endoglucanase transcripts in adult females could be related to the expression of the gene in esophageal glands at this stage. However, cellulase activity within the egg matrix of adult females suggests that the endoglucanase may also be synthesized in the rectal glands and involved in the extrusion of the eggs onto the root surface. The maximum identity of the predicted MI-ENG-1 catalytic domain with the recently cloned cyst nematode beta-1,4-endoglucanases is 52.5%. In contrast to cyst nematodes, M. incognita pre-parasitic J2 were not found to express a beta-1,4-endoglucanase devoid of a cellulose-binding domain.

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Citations

Apr 10, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Anh-Tung PhamZenglu Li
Apr 24, 2010·Journal of Molecular Evolution·Annelies HaegemanGodelieve Gheysen
May 3, 2005·International Journal for Parasitology·Guozhong HuangRichard S Hussey
May 30, 2002·Molecular and Biochemical Parasitology·Stéphanie JaubertMarie-Noëlle Rosso
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Jan 1, 2003·Molecular Plant Pathology·John T JonesAlison Prior
Mar 5, 2011·Bioinformatics·Celine VensEtienne G J Danchin
Jul 25, 2009·Journal of Experimental Botany·G DubreuilM N Rosso
Dec 1, 2010·Journal of Experimental Botany·Paulo VieiraMarie-Noëlle Rosso
Jul 5, 2012·Molecular Plant-microbe Interactions : MPMI·Borong LinJinling Liao
Aug 4, 2012·Molecular Plant-microbe Interactions : MPMI·M JaouannetM N Rosso
Jul 27, 2005·Molecular Plant-microbe Interactions : MPMI·M N RossoP Abad
Jul 27, 2005·Molecular Plant-microbe Interactions : MPMI·Qing ChenJohn T Jones
Feb 16, 2006·Molecular Plant-microbe Interactions : MPMI·Stéphanie JaubertMarie-Noëlle Rosso
May 6, 2006·Molecular Plant-microbe Interactions : MPMI·Makedonka Mitreva-DautovaGeert Smant
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