Isolation of a complete A1AO ATP synthase comprising nine subunits from the hyperthermophile Methanococcus jannaschii

Extremophiles : Life Under Extreme Conditions
Astrid LinglVolker Müller

Abstract

Archaeal A(1)A(O) ATP synthase/ATPase operons are highly conserved among species and comprise at least nine genes encoding structural proteins. However, all A(1)A(O) ATPase preparations reported to date contained only three to six subunits and, therefore, the study of this unique class of secondary energy converters is still in its infancy. To improve the quality of A(1)A(O) ATPase preparations, we chose the hyperthermophilic, methanogenic archaeon Methanococcus jannaschii as a model organism. Individual subunits of the A(1)A(O) ATPase from M. jannaschii were produced in E. coli, purified, and antibodies were raised. The antibodies enabled the development of a protocol ensuring purification of the entire nine-subunit A(1)A(O) ATPase. The ATPase was solubilized from membranes of M. jannaschii by Triton X-100 and purified to apparent homogeneity by sucrose density gradient centrifugation, ion exchange chromatography, and gel filtration. Electron micrographs revealed the A(1) and A(O) domains and the central stalk, but also additional masses which could represent a second stalk. Inhibitor studies were used to demonstrate that the A(1) and A(O) domains are functionally coupled. This is the first description of an A(1)A(O) ATPase pr...Continue Reading

References

Dec 29, 1978·Biochemical and Biophysical Research Communications·D O Mountfort
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 17, 1992·Biochimica Et Biophysica Acta·G Schäfer, M Meyering-Vos
Feb 14, 1990·European Journal of Biochemistry·E Scheel, G Schäfer
Aug 31, 1987·Biochemical and Biophysical Research Communications·L I HochsteinW Altekar
Jan 1, 1993·Journal of Bacteriology·K InatomiK Nakamura
Jan 1, 1996·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·K Steinert, S Bickel-Sandkötter
Jan 16, 1997·Biochimica Et Biophysica Acta·P Dimroth
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·E J BoekemaW N Konings
May 1, 1999·The Journal of Biological Chemistry·M Forgac
May 26, 1999·Journal of Bioenergetics and Biomembranes·V MüllerT Lemker
Sep 8, 1999·Microbiology and Molecular Biology Reviews : MMBR·G SchäferV Müller
Nov 4, 2000·Nature Structural Biology·R H Fillingame
Dec 15, 2000·Current Opinion in Structural Biology·D StockJ E Walker
Feb 21, 2002·The Journal of Biological Chemistry·Unal CoskunVolker Müller
Apr 5, 2002·Genome Research·James E GalaganBruce Birren
Aug 1, 1992·Photosynthesis Research·J P Gogarten, L Taiz

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Citations

Oct 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth WatersMichiel Noordewier
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ulf KüperHarald Huber
Sep 29, 2011·The Journal of Biological Chemistry·Duncan G G McMillanGregory M Cook
Sep 16, 2015·The Journal of Biological Chemistry·Soumya MohantyJ Sivaraman
Mar 7, 2014·Microbiology and Molecular Biology Reviews : MMBR·Christopher BräsenBettina Siebers
Aug 18, 2016·Science Advances·Mohammad T Mazhab-Jafari, John L Rubinstein
May 13, 2006·Journal of Bioenergetics and Biomembranes·Volker MüllerMichael Fritz
Aug 31, 2006·The FEBS Journal·Cornelia Schwarzenlander, Beate Averhoff
Mar 24, 2011·Quarterly Reviews of Biophysics·Stephen P MuenchMichael A Harrison
Dec 27, 2019·The FEBS Journal·Dennis Litty, Volker Müller
Jan 17, 2015·The Journal of Biological Chemistry·Florian MayerVolker Müller
Jan 16, 2009·Biochemical Society Transactions·Tillmann BurghardtReinhard Rachel
Mar 15, 2011·Journal of Molecular Biology·Malathy Sony Subramanian ManimekalaiGerhard Grüber

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