Jan 1, 1994

Isolation of a conditional suppressor of leucine auxotrophy in Saccharomyces cerevisiae

Microbiology
Jack A HeinemannJ Horecka

Abstract

Phenotypically and genotypically (leu2-3, 112) Leu- cells of Saccharomyces cerevisiae gave rise to small colonies on medium devoid of leucine. This only occurred on plates with a high density of Leu- cells or on medium supplemented with limiting quantities of leucine. Cells from these small colonies retained a growth advantage over their parent on limiting leucine supplements even after growth in a non-selecting (rich) medium. Therefore, the growth variants had acquired a heritable change. The phenotype was recessive and due to a change in a nuclear gene unlinked to the LEU2 locus. The phenotype provided a growth advantage only during leucine starvation; growth of the variants was indistinguishable from their parent on medium lacking the other essential supplements (histidine and uracil) required for the growth of the strain. [14C]Leucine uptake assays demonstrated that the variants were better able than their parents to accumulate leucine from their environments, and this ability extended to other hydrophobic amino acids. These results suggest that in the variants an amino acid uptake system has been derepressed rather than there having been reversion or extragenic suppression of the mutation in leucine biosynthesis. We design...Continue Reading

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Mentioned in this Paper

Histidine
DLEU2 wt Allele
Saccharomyces cerevisiae allergenic extract
CD8A wt Allele
Amino Acids, I.V. solution additive
T-CELL ANTIGEN LEU2 GENE
Conditions, Recessive Genetic
Visual Suppression
Genes, Fungal
Uracil

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