PMID: 6413823Jan 1, 1983Paper

Isolation of a DNA sequence related to several plasmids from Bacillus thuringiensis after a mating involving the Streptococcus faecalis plasmid pAM beta 1

Molecular & General Genetics : MGG
Didier LereclusM M Lecadet

Abstract

The transmissible plasmid pAM beta 1, which codes for resistance to erythromycin and lincomycin, was transferred from Streptococcus faecalis to several strains of Bacillus thuringiensis by a filter-mating process. Introduction of pAM beta 1 into the Emr transconjugant strains of B. thuringiensis was confirmed by Southern hybridisation using the 32P-labelled pAM beta 1 as a probe. In the B. thuringiensis transconjugant strains, used as donors, the beta plasmid conserved its ability to be transferred during intraspecific mating, with a frequency of 10(-4) per recipient cell. In addition, the transconjugant clones acted as donors of the erythromycin resistance marker and permitted the transfer of cryptic plasmids present in the B. thuringiensis (beta) strains used as donors. From a transconjugant clone of B. thuringiensis a hybrid plasmid resulting from an in vivo insertion into pAM beta 1 of a 3 Md DNA sequence was isolated. This 3 Md DNA molecule originated from a 54 Md plasmid of a kurstaki strain and is related to several plasmids found in different serotypes of B. thuringiensis.

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Citations

Jul 1, 1986·Molecular & General Genetics : MGG·D LereclusM M Lecadet
Nov 1, 1986·Molecular & General Genetics : MGG·P Courvalin, C Carlier
Mar 22, 2013·Genome Announcements·Guiming LiuFuping Song
Nov 1, 1985·Annales De L'Institut Pasteur. Microbiology·G Reysset, M Sebald
Jan 1, 1987·Critical Reviews in Biotechnology·R E AndrewsL A Bulla
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·E SchnepfD H Dean
Oct 1, 1987·Applied and Environmental Microbiology·D van der Lelie, G Venema
May 22, 2019·Microbiology Spectrum·Monika Ehling-SchulzTheresa M Koehler
Oct 1, 1984·Journal of Bacteriology·J W Kronstad, H R Whiteley
Sep 1, 1986·Applied and Environmental Microbiology·A W ShragoW J Dobrogosz
Apr 1, 1991·Applied and Environmental Microbiology·T M Walter, A I Aronson
Mar 1, 1986·Microbiological Reviews·A I AronsonP Dunn
Jun 9, 2016·Antimicrobial Agents and Chemotherapy·C A HardimanK M Frank
Jan 1, 1985·Biochimie·D LereclusR Dedonder

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