Isolation of a fission yeast mutant that is sensitive to valproic acid and defective in the gene encoding Ric1, a putative component of Ypt/Rab-specific GEF for Ryh1 GTPase.

Molecular Genetics and Genomics : MGG
Yan MaTakayoshi Kuno

Abstract

Valproic acid (VPA) causes various therapeutic and biological effects, but the exact mechanisms underlying these effects, however, remain elusive. To gain insights into the molecular mechanisms of VPA action, we performed in fission yeast a genetic screen for mutants that show VPA hypersensitivity and have identified several membrane-trafficking mutants including vas1-1/vps45 and vas2-1/aps1. Here, we describe the isolation and characterization of vas3-1/ric1-v3, a mutant allele of the ric1 (+) gene encoding a fission yeast homolog of the budding yeast Ric1p, a component of Ypt/Rab-specific guanyl-nucleotide exchange factor (GEF). The Rab GTPase Ryh1 knockout (Deltaryh1) cells and Deltaric1 cells exhibited similar phenotypes. The double knockout Deltaric1Deltaryh1 cells did not display synthetic growth defects. These results are consistent with the notion that Ric1 may be a component of the GEF complex for Ryh1. Overexpression of wild-type Ryh1 and the constitutively active Ryh1Q70L only partially suppressed the phenotypes of ric1-v3 and Deltaric1 cells, and they failed to localize to the Golgi/endosomes in ric1-v3 and Deltaric1 cells. Furthermore, we isolated vps15 (+) gene, encoding a serine/threonine protein kinase, as a dos...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Jan 1, 1983·Methods in Enzymology·R J Rothstein
Dec 1, 1994·The Journal of Cell Biology·O MartinezB Goud
Feb 23, 1995·Nature·R HeimR Y Tsien
Dec 1, 1994·Molecular and Cellular Biology·S M AlthoffJ A Wise
Aug 1, 1994·Yeast·S L Forsburg
Oct 8, 1998·Molecular Biology of the Cell·S JonesN Segev
May 18, 1999·The Journal of Biological Chemistry·H HamaB F Horazdovsky
Nov 13, 1999·Nature Cell Biology·S R Pfeffer
Sep 16, 2000·The EMBO Journal·S SiniossoglouH R Pelham
Oct 19, 2000·The Journal of Cell Biology·W WangS Ferro-Novick
Dec 5, 2000·Molecular Biology of the Cell·S JonesN Segev
Oct 2, 2001·Science's STKE : Signal Transduction Knowledge Environment·N Segev
Feb 5, 2003·Biochemical and Biophysical Research Communications·Masaki Edamatsu, Yoko Y Toyoshima
Aug 28, 2003·The Journal of Biological Chemistry·Banafshé LarijaniMiguel C Seabra
Feb 18, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yi HeTakayoshi Kuno
Dec 1, 2006·Journal of Molecular Biology·Aymelt ItzenRoger S Goody
Mar 11, 2008·Current Genetics·Alexander GeorgievAnn Mutvei
Jul 25, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yan MaTakayoshi Kuno
Sep 15, 2009·Brain Research Bulletin·Xiu-Zhen ZhangHong-Yu Zhang
Sep 17, 2009·Biochemical Society Transactions·Nicole Terbach, Robin S B Williams
Sep 24, 2009·Molecular and Cellular Endocrinology·N FortunatiG Boccuzzi
Dec 23, 2009·Cancer Biotherapy & Radiopharmaceuticals·Xufeng ChenJeffrey Y C Wong

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Citations

Apr 3, 2012·Annual Review of Biochemistry·Emi Mizuno-YamasakiPeter Novick
Nov 3, 2010·Current Biology : CB·Hisashi TatebeKazuhiro Shiozaki
Jun 4, 2016·Fungal Genetics and Biology : FG & B·Lingling HuYue Fang
Oct 27, 2016·Scientific Reports·Kristelle Desfossés-BaronHugo Wurtele
Oct 25, 2017·Frontiers in Microbiology·Julien ChaillotAdnane Sellam
May 23, 2018·Oxidative Medicine and Cellular Longevity·Teruaki TakasakiReiko Sugiura

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