Isolation of a galactose-binding lectin from the venom of the snake Bothrops godmani (Godmann's pit viper)

Toxicon : Official Journal of the International Society on Toxinology
B LomonteG Ramírez

Abstract

A galactose-binding lectin, isolated from the venom of B. godmani by affinity chromatography, is an acidic protein (pI 4.9) with a subunit mol. wt of about 14,000, occurring mostly as a disulfide-linked dimer of 28,000. A small proportion of lectin appears as a monomer and as a tetramer. The lectin agglutinates erythrocytes from mice, rabbit, cow and human (all ABO types, either Rh positive or negative), but does not agglutinate horse, sheep, goat and snake (Oxybelis aeneus, Colubridae) erythrocytes. The agglutinating activity is inhibited by 1 mM EDTA. The lectin is devoid of lethal, hemorrhagic, myotoxic, proteolytic and phospholipase A2 activities. It is not mitogenic for human peripheral blood mononuclear cells. The only effect observed was a moderate induction of edema in the footpad of mice, with a minimal edema-forming dose of 22 micrograms. This effect developed rapidly, and was significantly inhibited by i.p. administration of cyproheptadine, a histamine and serotonin antagonist, before injection of the lectin. Despite the edema-forming activity observed, the low concentration of lectin in crude venom, together with its relatively low potency, suggest that this lectin is not a key component in the development of edema ...Continue Reading

References

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Citations

Jul 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·S P Liang, X Pan
Nov 17, 2001·Toxicon : Official Journal of the International Society on Toxinology·Lachlan D Rash, Wayne C Hodgson
Jan 15, 1998·Toxicon : Official Journal of the International Society on Toxinology·F E RussellM C Fernandez
Sep 15, 1999·Toxicon : Official Journal of the International Society on Toxinology·Y KomoriH Sugihara
Sep 8, 2010·Toxicon : Official Journal of the International Society on Toxinology·Thatiane C AntunesMarcelo L Santoro
Oct 25, 2008·Toxicon : Official Journal of the International Society on Toxinology·F P SilvaS G De-Simone
Nov 3, 2004·Archives of Biochemistry and Biophysics·Viviane Guimarães-GomesRussolina B Zingali
May 30, 2006·Toxicon : Official Journal of the International Society on Toxinology·Marcus Davis Machado BragaHelena Serra Azul Monteiro
Sep 29, 2015·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Marco A Sartim, Suely V Sampaio
Nov 28, 2012·International Journal of Biological Macromolecules·Letícia Eulálio CastanheiraVeridiana M Rodrigues
Feb 22, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Erika dos Santos NunesMaria Tereza dos Santos Correia
Feb 8, 2011·Acta Biochimica Et Biophysica Sinica·Elaine de Paula Mendonça-FranqueiroSuely Vilela Sampaio
May 7, 2020·Journal of Proteome Research·Rongfeng LiPengcheng Li
Nov 7, 2007·Biochemical and Biophysical Research Communications·Akiko IguchiHiroshi Nagai

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