PMID: 4905956Mar 1, 1970Paper

Isolation of a Golgi apparatus-rich fraction from rat liver. I. Method and morphology

The Journal of Cell Biology
D J MorréV S Lequire

Abstract

Golgi apparatus were released without fixatives from rat hepatocytes by gentle homogenization, concentrated by differential centrifugation, and purified by sucrose gradient centrifugation. Examination of sections of purified fractions by electron microscopy showed fields of morphologically intact units of Golgi apparatus consisting of stacks of parallel flattened cisternae, secretory vesicles, and small vesicular profiles. Negative staining of unfixed pellets revealed a complex network of anastomotic tubules continuous with platelike structures and secretory vesicles. These structures corresponded, respectively, to the small vesicular profiles and parallel flattened cisternae with attached secretory vesicles of sectioned material. Small fragments of granular endoplasmic reticulum were often closely associated with the peripheral tubules, suggesting sites of continuity in intact hepatocytes.

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Citations

Apr 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G M Cook
Jan 1, 1977·Journal of Supramolecular Structure·B Fleischer
Jan 1, 1978·The International Journal of Biochemistry·W A Hughes, H G Coore
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Apr 30, 2013·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Yaying YangYongfen Yi
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Apr 1, 1988·Archives of Biochemistry and Biophysics·R Schüz-HenningerK Decker
Dec 4, 1972·Biochemical and Biophysical Research Communications·C LarsénL Ernster

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