PMID: 8445329Feb 1, 1993Paper

Isolation of a human endothelial cell C1q receptor (C1qR)

Journal of Leukocyte Biology
E I PeerschkeR B Sim

Abstract

C1q binding to endothelial cells has been described previously, but the putative cell surface receptor(s) has not been identified. In the present study, modifications of a reported purification of lymphocyte C1q receptor (C1qR) were used to isolate C1q binding sites from human umbilical vein endothelial cells. Cells were harvested, without protease treatment, at passage 10-17 and lysed with 1% Triton X-100. The lysate was fractionated on Fast-performance liquid chromatography (FPLC) Mono-Q using a linear NaCl gradient, followed by high-performance liquid chromatography (HPLC) ion exchange (TSKgel DEAE-NPR). A major protein was eluted that had the same mobility on sodium dodecyl sulfate-polyacrylamide gels and the same NH2-terminal sequence as lymphocyte C1qR. This protein was expressed on the surface, as judged by surface radioiodination, bound to C1q-coated surfaces, and was recognized by polyclonal antilymphocyte C1qR antibodies. Thus, endothelial cells express a C1q receptor that appears identical to lymphocyte C1qR. The data further support the hypothesis that cell surface C1qRs identified on a variety of somatic and cultured cells are either identical or constitute a family of closely related molecules.

Citations

Feb 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaodong FengBerhane Ghebrehiwet
Sep 27, 2019·Frontiers in Cardiovascular Medicine·Hugh Kim, Edward M Conway
Jul 1, 1997·American Journal of Respiratory Cell and Molecular Biology·A S NarayananG Raghu
Aug 31, 2000·The Journal of Biological Chemistry·S GoicoecheaJ E Murphy-Ullrich
Nov 21, 2019·Seminars in Immunology·Berhane GhebrehiwetEllinor I B Peerschke
Jun 1, 1997·Immunopharmacology·A P KaplanY Shibayama
Feb 27, 1999·Current Opinion in Immunology·A Nicholson-Weller, L B Klickstein
Jul 22, 2014·Molecular Immunology·Ellinor I B Peerschke, Berhane Ghebrehiwet
Feb 1, 1993·Current Opinion in Immunology·K Sastry, R A Ezekowitz
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