PMID: 6170338Sep 24, 1981Paper

Isolation of a major apolipoprotein of canine and murine pulmonary surfactant. Biochemical and immunochemical characteristics

Biochimica Et Biophysica Acta
K Sueishi, B J Benson

Abstract

We studied some of the biochemical and immunochemical properties of a major apolipoprotein in isolated pulmonary surfactant from dog and rat lungs. These apolipoproteins were purified by DEAE-cellulose chromatography in buffers containing Triton X-100. Purity of the apolipoproteins was assessed by both fused rocket and crossed immunoelectrophoreses. In addition, the apolipoproteins showed one band with an apparent molecular weight of 72 000-73 000 on SDS-polyacrylamide gel electrophoresis. These proteins are composed of two polypeptide chains of 36 000 daltons. When subjected to isoelectric focusing, the major component of the apolipoprotein had an isoelectric point of about 4.4, with very minor components near 4.6. Even though the apolipoproteins of both species had very similar amino acid compositions, including a relatively high glycine content, no immunologic cross-reactivity was observed. Rocket immunoelectrophoretic analysis of several preparations of dog and rat surfactant using the respective purified apolipoproteins as standards indicated that the apolipoprotein constituted 56.9% +/- 4.6. (S.D., n = 3) and 42.1% +/- 2.1 (S.D., n = 2) of the total protein in dog and rat surfactant, respectively.

References

Dec 1, 1979·The Biochemical Journal·P J PhizackerleyG E Newman
Apr 1, 1977·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·R J KingF M Holmstrom
Jul 22, 1977·Biochimica Et Biophysica Acta·H SawadaY Okajima
Jan 1, 1977·Preparative Biochemistry·J J MaguireJ U Balis
Jan 1, 1976·Preparative Biochemistry·G ColaciccoE M Scarpelli
Nov 1, 1975·Journal of Applied Physiology·R J KingR K Creasy
Mar 18, 1976·Biochimica Et Biophysica Acta·S N BhattacharyyaW S Lynn
Apr 1, 1971·Analytical Biochemistry·G R Finlayson
Jan 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·C B Laurell
Jun 1, 1973·Journal of Applied Physiology·G ColaciccoE M Scarpelli
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M A PasseroW S Lynn
Sep 1, 1974·The American Review of Respiratory Disease·R J KingJ A Clements
Oct 6, 1972·Biochemical and Biophysical Research Communications·D C MerzG W Litman
Dec 16, 1974·Biochimica Et Biophysica Acta·J Goerke
Apr 1, 1973·The American Journal of Physiology·R J KingJ A Clements
Mar 1, 1973·Archives of Biochemistry and Biophysics·P BöhlenS Udenfriend
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·P J Svendsen
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·O J Bjerrum, P Lundahl
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·N H Axelsen
Aug 9, 1974·Science·B BallouM L Bach
Sep 1, 1972·The American Journal of Physiology·R J King, J A Clements
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B J Benson
Feb 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M C Williams, B J Benson
Aug 1, 1964·The American Journal of Physiology·E S BROWN

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Citations

Sep 2, 1985·Klinische Wochenschrift·B Müller, P von Wichert
Jan 1, 1985·Molecular Aspects of Medicine·J L Harwood, R J Richards
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D S Phelps, H W Taeusch
Jan 1, 1988·General Pharmacology·T E Weaver
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B BensonR T White
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P L BallardR T White
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S R RannelsD E Rannels
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L G DobbsJ Nellenbogen
Jan 1, 1984·Experimental Lung Research·S L Katyal, G Singh
Jan 1, 1984·Experimental Lung Research·W S Lynn
Jan 1, 1984·Experimental Lung Research·B J BensonM C Williams
Jul 23, 2003·The Korean Journal of Internal Medicine·Chung Mi KimSung Soo Park
Jun 1, 1983·Bioscience Reports·R C CottrellK Miller
Jan 3, 1994·Biochimica Et Biophysica Acta·M J KreschC Christian
Jun 23, 2018·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·C I BassorgunO Kankavi
Apr 6, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·J F HerbeinJ R Wright
Mar 1, 1986·The American Journal of Physiology·R J KingJ Morrison-Plummer
Mar 22, 1983·Biochimica Et Biophysica Acta·D J McIverS Schürch
Jun 27, 1986·Biochimica Et Biophysica Acta·L G DobbsS Hawgood
Dec 16, 1988·Biochimica Et Biophysica Acta·R H LumbJ A Clements
Jan 13, 1987·Biochimica Et Biophysica Acta·J G NijssenH van den Bosch
Dec 4, 1985·Biochimica Et Biophysica Acta·A D PostleI C Normand
Mar 27, 1984·Biochimica Et Biophysica Acta·B J BensonT Sargeant
Jul 26, 1984·Biochimica Et Biophysica Acta·S L Katyal, G Singh
Dec 7, 1984·Biochimica Et Biophysica Acta·D S PhelpsS Hawgood
Mar 1, 1985·Biochimica Et Biophysica Acta·T E WeaverJ A Whitsett
Oct 14, 1982·Biochimica Et Biophysica Acta·L G DobbsK Sueishi

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