Oct 2, 2002

Isolation of a mutant strain of Pseudomonas sp ATCC 31461 exhibiting elevated polysaccharide production

Journal of Industrial Microbiology & Biotechnology
Thomas P West

Abstract

A mutant strain of the bacterium Pseudomonas sp. ATCC 31461 that exhibited elevated production of the polysaccharide gellan on glucose or corn syrup as a carbon source was isolated. Gellan production by the mutant strain was about twofold higher than its parent strain on glucose or corn syrup after 48 h of growth, and about 1.4-fold higher after 72 h. An increase in biomass production was not correlated with enhanced gellan synthesis by the mutant strain. The increased gellan production by the mutant strain on either carbon source resulted in an increase in its culture medium viscosity and the viscosity of the isolated polysaccharide produced by glucose-grown cells. No differences in the glucuronic acid content of the polysaccharides produced by the mutant and parent strains were observed.

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Mentioned in this Paper

Glucose, (beta-D)-Isomer
Polysaccharides, Bacterial
Viscosity
Pseudomonas species
Mutant
Glucuronic Acid
Corn Syrup
Pseudomonas
Blood Viscosity Examination
Biomass

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