Isolation of a new broad-host-range IncQ-like plasmid, pTC-F14, from the acidophilic bacterium Acidithiobacillus caldus and analysis of the plasmid replicon

Journal of Bacteriology
M N GardnerD E Rawlings

Abstract

A moderately thermophilic (45 to 50 degrees C), highly acidophilic (pH 1.5 to 2.5), chemolithotrophic Acidithiobacillus caldus strain, f, was isolated from a biooxidation process used to treat nickel ore. Trans-alternating field electrophoresis analysis of total DNA from the A. caldus cells revealed two plasmids of approximately 14 and 45 kb. The 14-kb plasmid, designated pTC-F14, was cloned and shown by replacement of the cloning vector with a kanamycin resistance gene to be capable of autonomous replication in Escherichia coli. Autonomous replication was also demonstrated in Pseudomonas putida and Agrobacterium tumefaciens LBA 4404, which suggested that pTC-F14 is a broad-host-range plasmid. Sequence analysis of the pTC-F14 replicon region revealed five open reading frames and a replicon organization like that of the broad-host-range IncQ plasmids. Three of the open reading frames encoded replication proteins which were most closely related to those of IncQ-like plasmid pTF-FC2 (amino acid sequence identities: RepA, 81%; RepB, 78%; RepC, 74%). However, the two plasmids were fully compatible and pTC-F14 represents a new IncQ-like plasmid replicon. Surprisingly, asymmetrical incompatibility was found with the less closely relat...Continue Reading

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Citations

Oct 26, 2005·Applied Microbiology and Biotechnology·Michael BramucciVasantha Nagarajan
Aug 2, 2002·Plasmid·Trine M L'Abée-Lund, Henning Sørum
Jul 29, 2008·Applied and Environmental Microbiology·Leonardo J van ZylDouglas E Rawlings
Sep 6, 2003·Applied and Environmental Microbiology·Carl-Alfred AlpertMonique Zagorec
Oct 5, 2010·Journal of Bacteriology·Wesley Loftie-Eaton, Douglas E Rawlings
Aug 18, 2009·Journal of Bacteriology·Wesley Loftie-Eaton, Douglas E Rawlings
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·D E Rawlings, E Tietze
Jan 22, 2014·Applied Microbiology and Biotechnology·Ming-Jiang ZhangShuang-Jiang Liu
Nov 1, 2011·Plasmid·Wesley Loftie-Eaton, Douglas E Rawlings
Jul 8, 2008·Journal of Biotechnology·Andreas SchlüterAlfred Pühler
Nov 9, 2011·Environmental Microbiology·Yingfei MaBrian Palenik
Jun 9, 2004·Microbiology·Murray N Gardner, Douglas E Rawlings

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