Isolation of a non-sedimenting membrane fraction from the water soluble fraction of bovine lens

Experimental Eye Research
Charles R Fleschner, R J Cenedella

Abstract

A membrane fraction was isolated from the water soluble fraction of bovine lens by increasing the density of the water soluble fraction with KBr and subjecting it to overnight centrifugation at 100000 g. We have called this fraction, which floats to the top of the mixture upon centrifugation, the non-sedimenting membrane fraction (NSMF). Electron microscopy of the NSMF revealed that it is composed of the expected membrane structures of unit membrane, fiber junction and cytoskeleton. Significantly less of the total membrane of the NSMF was devoted to fiber junction (22.8%) than in the sedimenting membrane fraction (SMF) (41.1%) prepared by sucrose density centrifugation of the water insoluble fraction. The NSMF accounted for about 7-12% of the total bovine lens membrane, and preliminary experiments demonstrated that a similar fraction could be isolated from the water soluble fraction of lenses from rats, rabbits, chickens and humans. The NSMF contained about 0.9 mg total lipid per mg total membrane protein, which was significantly greater than the value obtained for the SMF (0.5 mg total lipid per mg total membrane protein). The greater relative amount of total lipid in the NSMF was due to a significantly greater relative amount...Continue Reading

Citations

Nov 1, 1993·Current Eye Research·R J Cenedella, G Chandrasekher
Sep 30, 2005·Ophthalmic Research·C R Fleschner
Sep 27, 2002·Current Eye Research·Charles Ricky Fleschner
Mar 5, 2004·Chemistry and Physics of Lipids·M Cecilia Yappert, Douglas Borchman
Jan 1, 1996·Survey of Ophthalmology·R J Cenedella
Jun 1, 1995·Experimental Eye Research·G Chandrasekher, R J Cenedella

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