PMID: 11311858Apr 20, 2001Paper

Isolation of a novel heterodimeric agglutinin from rhizomes of Smilax glabra, the Chinese medicinal material tufuling

The International Journal of Biochemistry & Cell Biology
T B Ng, Y L Yu

Abstract

A heterodimeric agglutinin with a molecular mass of 32 kDa, and comprised of a 15 and a 17 kDa-subunit, was isolated from Smilax glabra rhizomes. The isolation protocol entailed ion exchange chromatography on CM-Sepharose, DEAE-cellulose and Resource Q and gel filtration on Superose 12. The two agglutinin subunits resembled each other and lectins from other Liliaceae plants in N-terminal sequence. The hemagglutinating activity of the agglutinin was unstable under acidic and alkaline conditions and when exposed to temperatures at or higher than 50 degrees C. The activity was not altered by a number of monovalent, divalent and trivalent cations, nor by a variety of sugars and glycoproteins.

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Citations

Mar 26, 2003·Protein Expression and Purification·H X Wang, T B Ng
Jun 13, 2009·Natural Product Research·Antoaneta IvanovaIvanka Kostova
Jun 29, 2010·Journal of Ethnopharmacology·Daozong XiaWei Ma
Dec 30, 2008·Toxicon : Official Journal of the International Society on Toxinology·J K ZhaoT B Ng
Jun 16, 2010·Journal of the Science of Food and Agriculture·Wichchulada KonkumnerdPolkit Sangvanich
Oct 12, 2004·The International Journal of Biochemistry & Cell Biology·S Tripathi, Tapas K Maiti
May 30, 2006·Biochemical and Biophysical Research Communications·K FengH X Wang
Sep 6, 2005·Archives of Biochemistry and Biophysics·Qiaojuan YanLujia Han
Sep 2, 2015·Molecular & Cellular Proteomics : MCP·Antje GroscheStefanie M Hauck
Feb 13, 2018·The American Journal of Chinese Medicine·Shiyao HuaXueyan Fu

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