Isolation of a Novel Insect-Specific Flavivirus from Culiseta melanura in the Northeastern United States

Vector Borne and Zoonotic Diseases
Michael J MisencikPhilip M Armstrong

Abstract

The genus Flavivirus includes a number of newly recognized viruses that infect and replicate only within mosquitoes. To determine whether insect-specific flaviviruses (ISFs) may infect Culiseta (Cs.) melanura mosquitoes, we screened pools of field-collected mosquitoes for virus infection by RT-PCR targeting conserved regions of the NS5 gene. NS5 nucleotide sequences amplified from Cs. melanura pools were genetically similar to other ISFs and most closely matched Calbertado virus from Culex tarsalis, sharing 68.7% nucleotide and 76.1% amino acid sequence identity. The complete genome of one virus isolate was sequenced to reveal a primary open reading frame (ORF) encoding a viral polyprotein characteristic of the genus Flavivirus. Phylogenetic analysis showed that this virus represents a distinct evolutionary lineage that belongs to the classical ISF group. The virus was detected solely in Cs. melanura pools, occurred in sampled populations from Connecticut, New York, New Hampshire, and Maine, and infected both adult and larval stages of the mosquito. Maximum likelihood estimate infection rates (MLE-IR) were relatively stable in overwintering Cs. melanura larvae collected monthly from November of 2012 through May of 2013 (MLE-IR ...Continue Reading

References

Jan 1, 1989·Advances in Virus Research·T W Scott, S C Weaver
Jan 7, 1998·Journal of Virology·G KunoC B Cropp
Feb 1, 2005·Vector Borne and Zoonotic Diseases·Theodore G AndreadisAndrew J Main
Dec 9, 2008·Journal of Medical Entomology·M E Morales-BetoulleB W Johnson
Sep 4, 2009·Journal of Virology·Gang ZouPei-Yong Shi
Jan 1, 2007·Journal of Insect Science·Joel J L LutomiahRosemary C Sang
Apr 8, 2010·Vector Borne and Zoonotic Diseases·Jose A Farfan-AleBradley J Blitvich
May 11, 2010·PLoS Neglected Tropical Diseases·Rebekah J KentBarry R Miller
Jan 25, 2011·Vector Borne and Zoonotic Diseases·Christina M NewmanTony L Goldberg
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Jul 8, 2011·The American Journal of Tropical Medicine and Hygiene·Bethany G BollingCarol D Blair
Sep 23, 2011·Journal of Medical Entomology·Rungrat SaiyasombatBradley J Blitvich
Oct 21, 2011·The Journal of General Virology·Shelley CookRalph E Harbach
Feb 9, 2012·Journal of Medical Entomology·Rebekah Kent CrockettRoger Nasci
Aug 3, 2012·Intervirology·Daiane Cristina MachadoMauricio Lacerda Nogueira
Aug 14, 2013·The Journal of General Virology·Jolanta KolodziejekNorbert Nowotny

❮ Previous
Next ❯

Citations

Oct 11, 2017·The American Journal of Tropical Medicine and Hygiene·Hilda GuzmanRobert B Tesh
Sep 20, 2019·Viruses·Eric AgboliEsther Schnettler
Jul 19, 2017·The American Journal of Tropical Medicine and Hygiene·Maria Angelica Contreras-GutierrezRobert B Tesh
Jun 30, 2018·Virology·James Weger-LucarelliDoug E Brackney

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
RNA-Seq

Software Mentioned

Gblocks
Trinity
MEGA
ClustalW
Geneious
Excel Add - In PooledInfRate

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.