PMID: 16520318Mar 8, 2006Paper

Isolation of a novel peptide that binds to BLyS from a 12-mer phage display peptide library

Yi chuan = Hereditas
Yan-Li DingYi Gong

Abstract

A 12-mer phage display peptide library was screened for specific binders against B lymphocyte stimulator (BLyS). After 3 rounds of panning, positive phage clones were enriched. ELISAs were used to identify positive phages, and the DNA encoding the positive peptide (RHKIQLRQNIIT) was cloned and expressed as a GST fusion protein in E. coli. After purification, the identity of the fusion protein was confirmed through its specific binding to BLyS by ELISA. We have obtained a peptide that can bind to BLyS and probably act as an antagonistic peptide against the natural BLyS receptor.

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Related Papers

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Lihua HouWei Chen
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Qing JiHan-qi Zheng
Methods in Molecular Biology
Bao Guo-Qiang, He Xian-Li
© 2021 Meta ULC. All rights reserved