Isolation of a novel promoter for efficient protein production in Aspergillus oryzae

Bioscience, Biotechnology, and Biochemistry
Hiroki IshidaYutaka Kashiwagi

Abstract

A novel protein overexpression system of Aspergillus oryzae was constructed. Five promoters which originate from A. oryzae expressed sequence tag (EST) clones in submerged culture were obtained by genome walking. These were subjected to beta-glucuronidase (GUS) reporter assays. The promoter of manganese superoxide dismutase-encoding gene (sodM) showed the most GUS production. The sodM gene was abundantly expressed in submerged culture but little expressed in solid-state culture. The sodM promoter was approximately 3-fold induced by the addition of 0.01% H2O2. Glucoamylase production in A. oryzae using the sodM promoter led to secretion of approximately 1 g/l-broth in Czapek-Dox medium for 3 d. Fucose lectin production in A. oryzae using the sodM promoter led to overexpression as a specific and abundant intracellular protein.

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Citations

Jun 3, 2014·Microbial Cell Factories·Ryosuke YamadaAkihiko Kondo
Jan 8, 2008·Bioscience, Biotechnology, and Biochemistry·Hiromoto HisadaMasayuki Machida
May 24, 2006·Bioscience, Biotechnology, and Biochemistry·Hiroki IshidaYasuhisa Abe
Jun 2, 2007·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Takeshi AkaoOsamu Akita
Aug 2, 2005·Applied Microbiology and Biotechnology·Akio KodaMasato Hirotsune
Mar 21, 2006·Applied Microbiology and Biotechnology·Hiromoto HisadaMasayuki Machida
Sep 25, 2008·Applied Microbiology and Biotechnology·Takashi AdachiAkihiko Kondo
Jun 10, 2010·Applied Microbiology and Biotechnology·André Fleissner, Petra Dersch
Jul 7, 2011·Applied Microbiology and Biotechnology·Hiroki BandoAkihiko Kondo
Apr 1, 2009·Journal of Bioscience and Bioengineering·Mayumi MatsushitaYutaka Kashiwagi
Oct 9, 2008·Biotechnology Advances·David Lubertozzi, Jay D Keasling
Nov 19, 2011·FEMS Microbiology Letters·Ruchika SharmaGhulam Nabi Qazi
Apr 27, 2021·Journal of Bioscience and Bioengineering·Taku SakuragawaAkihiko Kondo

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