Isolation of a pluripotent neural stem cell from the embryonic bovine brain

International Journal of Molecular Sciences
Yuhua GaoYuehui Ma

Abstract

We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells (NSCs) not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes).

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Citations

Mar 20, 2021·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Panpan XuJun Zheng

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Methods Mentioned

BETA
mechanical dissociation
PCR
electrophoresis

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