Abstract
We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells (NSCs) not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes).
References
Jan 1, 1989·Growth Factors·N EmotoA Baird
Jun 1, 1965·The Journal of Comparative Neurology·J Altman, G D Das
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C Lois, A Alvarez-Buylla
Feb 7, 1997·Cell·S J MorrisonD J Anderson
Jan 1, 1997·Molecular and Cellular Neurosciences·T D PalmerF H Gage
Mar 21, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D J Riese, D F Stern
Mar 17, 1999·Developmental Biology·V TropepeD van der Kooy
Apr 8, 1999·Experimental Neurology·A L VescoviR Galli
Dec 22, 1999·Journal of Biomedical Materials Research·E KonR Quarto
Aug 3, 2000·Stem Cells·S F PaganoE A Parati
Aug 31, 2002·Tissue Engineering·Yanchun LiuYilin Cao
Dec 12, 2003·Neuroscience·E M ParkS Cho
May 18, 2004·Experimental Hematology·Darko BosnakovskiToru Fujinaga
Feb 16, 2005·Developmental Neuroscience·Wei ZhengFlora M Vaccarino
Dec 22, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Chiu-Ming WenChung-Shung Wang
Oct 23, 2012·Molecular and Cellular Neurosciences·Philipp SütterlinGareth Williams
Mar 28, 2013·International Journal of Medical Sciences·Nor Entan SupenoHasnan Jaafar
Mar 8, 2014·Stem Cells Translational Medicine·Miriam E van StrienElly M Hol
Mar 19, 2014·Journal of Visualized Experiments : JoVE·Tara L Walker, Gerd Kempermann
Jul 30, 2014·Frontiers in Pediatrics·Antonella Peruffo, Bruno Cozzi
Citations
May 24, 2016·Neural Regeneration Research·Lei ZhangXin-Hua Zhang
Dec 19, 2016·Neuroscience Letters·Feifan XiangYuanlin Sun
Mar 20, 2021·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Panpan XuJun Zheng