PMID: 6168773Aug 1, 1981Paper

Isolation of a replication-efficient mutant of West Nile virus from a persistently infected genetically resistant mouse cell culture

Journal of Virology
M A Brinton

Abstract

Flavivirus-resistant mouse embryofibroblasts (C3H/RV) that were infected with West Nile virus, strain E101 (WNV), yielded fewer infectious virions than did cultures of congenic susceptible cells (C3H/HE). Analysis of intracellular viral RNA synthesis indicated that the incorporation of [3H]uridine into 40S genome RNA was markedly reduced in resistant cells, and about 100-fold less labeled 40S RNA was found in pelleted extracellular virions from resistant cultures than in those from susceptible ones. A non-temperature-sensitive mutant of WNV isolated from culture fluid of a persistently infected culture of genetically resistant mouse cells was found to produce higher yields of infectious virus than the parental WNV used to initiate the persistent infection. Analysis of intracellular actinomycin D-resistant RNA indicated that the mutant virus (WNV-RV) was more efficient at incorporating [3H]uridine into 40S RNA in resistant cells than was the parental virus. WNV-RV also synthesized 40S RNA more efficiently than parental virus in congenic susceptible cells and in BHK cells. Analysis of the incorporation of [35S]methionine into viral proteins was likewise enhanced in WNV-RV-infected cells. The WNV-RV mutant provides a tool for stud...Continue Reading

References

Mar 1, 1979·The Journal of Experimental Medicine·O HallerJ Lindenmann
Jan 1, 1977·Archives of Virology·E G WestawayL G McLeod
Sep 1, 1976·The Journal of General Virology·J P Clewley, S I Kennedy
Apr 1, 1969·Journal of Virology·D W TrentA A Qureshi
Apr 1, 1980·Journal of Virology·D L Sawicki, S G Sawicki
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Jun 1, 1952·Proceedings of the National Academy of Sciences of the United States of America·A B Sabin
Jan 31, 1937·The Journal of Experimental Medicine·L T Webster

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Citations

Sep 20, 2011·Frontiers in Biology·Lihui LiuHongmin Li
Apr 5, 2005·Journal of Neurovirology·Elizabeth Hunsperger, John T Roehrig
Nov 19, 2013·Virologica Sinica·Matthew BrecherHongmin Li
Jun 8, 2014·Archives of Virology·Tariq A MadaniHussein M S Al-Bar
Jul 27, 2002·Annual Review of Microbiology·Margo A Brinton
Apr 1, 1982·Infection and Immunity·M A BrintonO Haller

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