PMID: 8444161Feb 15, 1993Paper

Isolation of a ripening and wound-induced cDNA from Cucumis melo L. encoding a protein with homology to the ethylene-forming enzyme

European Journal of Biochemistry
C BalaguéD Grierson

Abstract

A cDNA clone (pMEL1) was isolated from a climacteric melon fruit cDNA library using the tomato ethylene-forming-enzyme (EFE) cDNA, pTOM13, as a probe. Northern analysis of RNA isolated from wounded leaf and fruit tissue and from preclimacteric and climacteric pericarp tissue was used to determine the pattern of pMEL1 RNA expression. pMEL1 hybridized to a 1.3-kb transcript in climacteric fruit and wounded leaf tissue but was undetectable in preclimacteric fruit and unwounded leaves. Maximal expression of pMEL1 RNA occurred in wounded ripe fruit. pMEL1 contained a 1230-bp insert containing a predicted open reading frame of 318 amino acids and molecular mass of 35.3 kDa. The predicted amino acid sequence of pMEL1 was 73-81% identical to the deduced amino acid sequences of tomato (pTOM13) EFE and EFE-related genes isolated from tomato and avocado fruit and senescent carnation petals. Genomic Southern analysis indicated that pMEL1 hybridized to a number of genomic fragments consistent with the presence of more than one pMEL1-related gene in melon. On Western analysis of total protein extracts from ripe tomato and melon fruit, using an antibody raised against tomato EFE (pTOM13) expressed in Escherichia coli, a single 35.5-kDa protei...Continue Reading

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Citations

Mar 1, 1995·Plant Molecular Biology·Z KarvouniD Grierson
Apr 24, 1996·Molecular & General Genetics : MGG·E LasserreC Balagué
Feb 24, 2001·Genome Génome / Conseil National De Recherches Canada·Y Danin-PolegN Katzir
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Andy G. Prescott, Philip John
Feb 16, 2002·Journal of Experimental Botany·Hidenori TakahashiHiroshi Ezura
Jul 1, 1996·Nature Biotechnology·D Grierson
Jun 1, 2002·Plant & Cell Physiology·Mei-Chu ChungShang Fa Yang

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