Isolation of a stromal cell line from an early passage of a mouse mammary tumor line: a model for stromal parenchymal interactions

Journal of Cellular Physiology
Caroline A LambClaudia Lanari

Abstract

We have developed a murine mammary tumor cell line, MC4-L4, and after 15 passages, a spindle-shaped population became evident. The cuboidal cells, MC4-L4E, cloned by limit dilution, proved to be epithelial tumor cells. When inoculated in syngeneic mice, they gave rise to invasive metastatic carcinomas expressing estrogen and progesterone receptors. These tumors regressed after anti-progestin treatment and stopped growing after 17-beta-estradiol administration. In vitro, they were insensitive to medroxyprogesterone acetate (MPA), 17-beta-estradiol, and EGF and were inhibited by TGFbeta1. They expressed mutated p53 and estrogen receptors alpha; progesterone receptors were undetectable. Cells were polyploid and shared the same four common marker chromosomes present in the parental tumor in addition to an exclusive marker. Spindle-shaped cells, MC4-L4F, were selected by differential attachment and detachment and proved to be non-epithelial non-tumorigenic cells. They were cytokeratin negative, showed mesenchymal features by electron microscopy, differentiated to adipocytes when treated with an adipogenic cocktail, were stimulated by TGFbeta1 and EGF, showed a wild-type p53, and did not exhibit the marker chromosomes of the parental...Continue Reading

References

Dec 2, 1991·International Journal of Cancer. Journal International Du Cancer·E KordonC Dosne Pasqualini
Jul 1, 1991·The Journal of Clinical Endocrinology and Metabolism·C MaletP Mauvais-Jarvis
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J L CampsL W Chung
May 15, 1989·International Journal of Cancer. Journal International Du Cancer·C LanariE H Charreau
Oct 30, 1971·Lancet·M Seabright
Apr 24, 1999·The Journal of Steroid Biochemistry and Molecular Biology·M F MontecchiaC Lanari
Aug 10, 2000·Journal of Mammary Gland Biology and Neoplasia·W P Bocchinfuso, K S Korach
Feb 13, 2001·Current Opinion in Genetics & Development·T D Tlsty, P W Hein
Oct 12, 2001·The Journal of Steroid Biochemistry and Molecular Biology·F KoernerH Maluf
Mar 20, 2002·Nature Reviews. Cancer·M J Bissell, D Radisky
Jun 5, 2002·Archiv der Pharmazie·Günther BernhardtHelmut Schönenberger
Jan 28, 2003·The American Journal of Pathology·Ole William PetersenLone Rønnov-Jessen
Jul 23, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Brenda H WelterThomas M Price
Aug 20, 2003·International Journal of Cancer. Journal International Du Cancer·Gerald R CunhaWilliam A Ricke
Feb 6, 2004·Breast Cancer Research and Treatment·Alejo EfeyanAlfredo A Molinolo

❮ Previous
Next ❯

Citations

Sep 4, 2008·International Journal of Cancer. Journal International Du Cancer·Sebastián GiulianelliClaudia Lanari
Jun 28, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M Luisa DóriaLuisa A Helguero

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.