PMID: 6168445Jan 1, 1981Paper

Isolation of a structural gene mapping to subregions 63F of Drosophila melanogaster and 90B of D. hydei polytene chromosomes

Chromosoma
M IzquierdoC Alonso

Abstract

We have isolated by molecular cloning techniques a structural gene that maps to subregion 63F of Drosophila melanogaster chromosome 3L. This locus being one of the early chromosomal targets for ecdysone stimulation, may be induced to puff by the hormone. The gene is on a plasmid vector and it has been designated as pDm 63F. This recombinant molecule also maps to the early ecdysone inducible subregion 90B of Drosophila hydei chromosome 4. The cytological inspection of large number of chromosomal sets after in situ hybridization of the cloned DNA, locates the cloned sequence between bands 63F 2-4 according to Bridges map. Similarly, in Drosophila hydei the cloned DNA maps between subdivisions 90B 2-4 according to Berendes' map. In situ hybridization of the pDm 63F cloned DNA, directed to the nascent RNA rather than to the DNA, shows two to three times more silver grains over the corresponding regions when puffed than in the resting stage. There are however quantitative differences attending to the transcriptional activity of homologous loci in both species. By RNA excess hybridization we have found that the cellular concentration of the 63F cloned mRNA is bout 2 times higher in hormone stimulated total larval tissues than in non-...Continue Reading

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K J LivakM Meselson
Jun 1, 1979·Biochemical Genetics·M Izquierdo, J O Bishop
Dec 20, 1979·Nucleic Acids Research·J P GergenP C Wensink
Jun 8, 1979·Science·E H Davidson, R J Britten
Jan 1, 1974·Annual Review of Genetics·G Lefevre
Jan 1, 1972·Results and Problems in Cell Differentiation·M Ashburner
Aug 1, 1974·Developmental Biology·D W BorstJ W Fristrom
Jan 1, 1973·Annual Review of Genetics·C D Laird
Dec 1, 1980·Cell·C D Laird
Jun 12, 1980·Nature·M Ashburner
Oct 24, 1980·Nucleic Acids Research·H Weintraub
Jan 1, 1974·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·I F Zhimulev, E S Belyaeva

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Citations

May 1, 1995·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·S C Lakhotia, A Sharma

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