PMID: 371687Mar 27, 1979

Isolation of a thiamine-binding protein from Saccharomyces cerevisiae

Biochimica Et Biophysica Acta
A Iwashima, H Nishimura

Abstract

Thiamine-binding protein was isolated from Saccharomyces cerevisiae by successive procedures of cold osmotic shock treatment, DEAE-cellulose chromatography and ultrafiltration. The purified thiamine-binding protein was an electrophoretically homogeneous molecule which appeared to be a glycoprotein with a molecular weight of 140 000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis. No thiamine-binding protein was observed by disc gel electrophoresis in the shock fluid released from yeast cells grown in the presence of 1 muM thiamine, indicating that the formation of this protein is regulated by exogenous thiamine as previously suggested.

Citations

Jun 15, 1986·Experientia·H NishimuraA Iwashima
Jun 1, 1984·Analytical Biochemistry·H NishimuraA Iwashima
Oct 1, 1988·Archives of Biochemistry and Biophysics·H NishimuraA Iwashima
Nov 2, 1979·Biochimica Et Biophysica Acta·A IwashimaY Nose
Jun 26, 1986·Biochimica Et Biophysica Acta·K NosakaA Iwashima
Jan 28, 2014·Biochemistry·Sergio ManzettiDavid van der Spoel
Jan 1, 1982·Annals of the New York Academy of Sciences·A M Hoyumpa

Related Concepts

Carrier Proteins
Saccharomyces cerevisiae
Thiamine Mononitrate

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