Isolation of a Trypanosoma cruzi antigen by affinity chromatography with a monoclonal antibody. Preliminary evaluation of its possible applications in serological tests

Clinical and Experimental Immunology
C H CarbonettoR A Margni

Abstract

By affinity chromatography with a monoclonal antibody (163B6), obtained in our laboratory, we have isolated a T. cruzi antigen which could be useful for differential diagnosis of Chagas' disease from leishmaniasis. This antigen, a 52-kD protein, reacted with all sera from Chagas' disease patients tested but not with sera from patients with leishmania, in ELISA. The 52-kD antigen is widely distributed in the Trypanosoma genus since the 163B6 monoclonal antibody reacts with T. rangeli and 8 strains and a clone of T. cruzi epimastigotes.

References

May 12, 1989·Journal of Immunological Methods·D I Stott
Jun 1, 1986·Experimental Parasitology·L A MullerS M González Cappa
Jan 1, 1986·Transactions of the Royal Society of Tropical Medicine and Hygiene·R C WongP J Hindmarsh
Jan 1, 1987·Transactions of the Royal Society of Tropical Medicine and Hygiene·S Giovanni-De-SimoneB Galvao-Castro
Jul 1, 1985·The American Journal of Tropical Medicine and Hygiene·D Chao, D G Dusanic
Sep 1, 1985·Molecular and Biochemical Parasitology·E A DragonJ Manning
Nov 1, 1985·The American Journal of Tropical Medicine and Hygiene·J ScharfsteinL Mendonça-Previato
Nov 1, 1973·The American Journal of Tropical Medicine and Hygiene·B C WaltonO Eguia y Eguia
Oct 1, 1974·The Journal of protozoology·E L SeguraJ A Cerisola
Jul 1, 1984·The American Journal of Tropical Medicine and Hygiene·O OrozcoA Capron
Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·P DesjeuxS Mollinedo

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Citations

Jan 1, 1993·Vox Sanguinis·S Wendel, A L Gonzaga
Aug 17, 2004·Transactions of the Royal Society of Tropical Medicine and Hygiene·N J TarantoE L Malchiodi
Jan 7, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Augusto E BivonaEmilio L Malchiodi
Sep 1, 2021·Journal of Medicinal Chemistry·Elany Barbosa da SilvaRafaela S Ferreira

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