Isolation of a Y chromosomal DNA sequence and its clinical application

Jinrui idengaku zasshi. The Japanese journal of human genetics
M TsukaharaT Kajii

Abstract

A 4.6 kb long, Y-specific DNA fragment was isolated from a flow-sorted human Y chromosomal library, and its male specificity was confirmed by Southern blot analysis. The fragment, designated as pY-80, was proven with an in situ hybridization experiment to have originated from the Yp11.2-Ypter region. Its 2,808 bp section was sequenced. The polymerase chain reaction proceeded with oligonucleotides flanking a 666 bp PstI-EcoRI fragment of the sequence as primers and a male genomic DNA as a template, but not with a female genomic DNA. Preliminary tests of samples of various sources successfully detected the Y-specific fragment in male-derived samples, including mouth wash, single hair roots, urinary epithelial cells, dried blood spots and amniotic fluid cells.

References

Nov 11, 1987·Nucleic Acids Research·J ArnemannJ Schmidtke
Jan 1, 1985·Human Genetics·M Tsukahara, T Kajii
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B U ZabelT B Shows
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A R Wyman, R White

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Citations

May 1, 1993·British Journal of Urology·H TakiharaT Kajii
Sep 1, 1992·The Japanese Journal of Human Genetics·S NagafuchiY Nakagome

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