Isolation of acholeplasmas and a mycoplasma from vegetables.

Applied and Environmental Microbiology
N L SomersonR A Del Giudice

Abstract

The isolation of Mollicutes from food has not been reported. To isolate Mollicutes in the presence of high levels of unwanted bacteria, we first incubated fresh vegetables in liquid culture media containing lysozyme, ampicillin, and thallous acetate. Culture fluids were than separated from the vegetable samples, subjected to one freeze-thaw cycle, and passed through a filter of 0.4-micron porosity. Filtered samples were cultured in SP4 medium and in a conventional medium containing horse serum. With this procedure 21 acholeplasma isolations representing three species were obtained from endive, broccoli, and kale. Of 35 food samples tested, 11 were positive for acholeplasmas; acholeplasmas isolated from 6 of these samples were recovered only in SP4 medium. In seven single vegetable samples, two or more Acholeplasma spp. were isolated. A. laidlawii was isolated from all three vegetables and A. axanthum was found in broccoli and kale. Four isolates were serologically identified as A. oculi. Mycoplasma verecundum was the only Mycoplasma species recovered. Several isolates could not be typed serologically, as they reacted with antisera to both A. morum and A. hippikon. these isolates may include new Acholeplasma spp.

References

Apr 1, 1979·The Journal of Infectious Diseases·J G TullyR P Wenzel
Apr 1, 1967·Journal of Bacteriology·R A Del GiudiceT R Carski
Jul 1, 1966·American Journal of Epidemiology·R H PurcellR M Chanock
Jan 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·R M CHANOCKM F BARILE
Jun 6, 1975·Science·D L Williamson, R F Whitcomb

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Citations

Jul 16, 2014·The Analyst·Omar E Rivera-BetancourtRichard A Dluhy
Feb 1, 1986·Applied and Environmental Microbiology·R G Cluss, N L Somerson
Jul 31, 1987·Science·K J HackettR F Whitcomb

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