Isolation of adult rhesus neural stem and progenitor cells and differentiation into immature oligodendrocytes.

Stem Cells and Development
Scott F DavisBruce A Bunnell

Abstract

Neural stem and progenitor cells (NSPCs) have been isolated from several regions of the brain from mice, rats, and humans. These cells possess the characteristics of self-renewal and differentiation along all major neural lineages. Herein, the first isolation of NSPCs from the adult rhesus macaque brain and characterization of these cells based on their gene and protein expression profile, self-renewal, and ability to differentiate along an oligodendrocyte lineage are described. Flow cytometric analysis revealed that this cell population is CD90(+)/CD164(+)/CD34(-) and, therefore, resembles a nonhematopoietic stem cell population. Similar to other mesenchymal and neural stem cell populations, rhesus NSPCs cells express stemness-related genes, including the transcription factors Oct-4, Rex-1, and Sox-2 and the gene encoding for the intermediate filament protein nestin. The co-expression of the neural and glial markers MAP2ab, GFAP, NF-L, and NeuroD was also observed at both the mRNA and protein levels. When rhesus NSPCs were induced to differentiate with a cocktail of retinoic acid and the neurotrophins (NGF, BDNF, and NT-3), they underwent morphologic changes including taking on an oligodendrocyte precursor morphology. Along wi...Continue Reading

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Citations

Jun 24, 2011·European Journal of Nuclear Medicine and Molecular Imaging·Jiachuan WangHong Zhang
Feb 21, 2008·American Journal of Nephrology·Sandeep Gupta, Mark E Rosenberg
Nov 20, 2014·Cell Regeneration·Guangming Wu, Hans R Schöler
Oct 1, 2011·Current Eye Research·Mikk PauklinDaniel Meller
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Dec 27, 2007·Cell Stem Cell·Christopher J LengnerRudolf Jaenisch
Jul 11, 2012·Cell Transplantation·Noélle D GermainLaura B Grabel

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