Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica

Frontiers in Microbiology
Guilherme L PinheiroSusana Frasés

Abstract

The enzymatic hydrolysis of cellulose by cellulases is one of the major limiting steps in the conversion of lignocellulosic biomass to yield bioethanol. To overcome this hindrance, significant efforts are underway to identify novel cellulases. The snail Achatina fulica is a gastropod with high cellulolytic activity, mainly due to the abundance of glycoside hydrolases produced by both the animal and its resident microbiota. In this study, we partially assessed the cellulolytic aerobic bacterial diversity inside the gastrointestinal tract of A. fulica by culture-dependent methods and evaluated the hydrolytic repertoire of the isolates. Forty bacterial isolates were recovered from distinct segments of the snail gut and identified to the genus level by 16S rRNA gene sequence analysis. Additional phenotypic characterization was performed using biochemical tests provided by the Vitek2 identification system. The overall enzymatic repertoire of the isolated strains was investigated by enzymatic plate assays, containing the following substrates: powdered sugarcane bagasse, carboxymethylcellulose (CMC), p-nitrophenyl-β-D-glucopyranoside (pNPG), p-nitrophenyl-β-D-cellobioside (pNPC), 4-methylumbelliferyl-β-D-glucopyranoside (MUG), 4-methy...Continue Reading

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Citations

Jul 26, 2016·Frontiers in Microbiology·Jatoth BalsinghKandasamy Ulaganathan
Jul 1, 2017·Microbiology and Molecular Biology Reviews : MMBR·Marco AgostoniMichael A Marletta
Jul 28, 2016·Applied Biochemistry and Biotechnology·Lijuan SuAndong Song
May 10, 2020·World Journal of Microbiology & Biotechnology·Jorge L González-EscobarAna P Barba de la Rosa
May 28, 2020·Frontiers in Microbiology·Carmen KivistikDaniel P R Herlemann
Jul 19, 2021·Animal Microbiome·Bridget Chalifour, Jingchun Li

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Datasets Mentioned

BETA
KF530754
KF530793
AB646581.2
AB166888.1

Methods Mentioned

BETA
light microscopy

Software Mentioned

Basic Local Alignment Search Tool ( BLAST )
Leica Application Suite
Vitek2
Blast
MEGA
Image Lab
MUSCLE

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