Isolation of alkaliphilic bacteria for production of high optically pure L-(+)-lactic acid

The Journal of General and Applied Microbiology
Hiroto Yokaryo, Yutaka Tokiwa

Abstract

Lactic acid bacteria that grow under alkaline conditions (pH 10) were isolated from various sources in Okinawa (Japan). These alkali-tolerant and alkaliphilic bacteria were classified as follows: Microbacterium sp. (1 strain), Enterococcus spp. (9 strains), Alkalibacterium spp. (3 strains), Exiguobacterium spp. (5 strains), Oceanobacillus spp. (3 strains) and Bacillus spp. (7 strains) by 16S rRNA gene sequencing. By fermentation, many strains were able to convert glucose into mainly L-(+)-lactic acid of high optical purity in alkaline broth. This result indicated that valuable L-(+)-lactic acid-producing bacteria could be isolated efficiently by screening under alkaline conditions. Six strains were selected and their ability to produce lactic acid at different initial pH was compared. Enterococcus casseliflavus strain 79w3 gave the highest lactic acid concentration. Lactic acid concentration and productivity were 103 g L(-1) (optical purity of 99.5% as L-isomer) and 2.2 g L(-1) h(-1), respectively when 129 g L(-1) of glucose was used by batch fermentation.

References

Jun 21, 2001·International Journal of Systematic and Evolutionary Microbiology·S Ntougias, N J Russell
Jun 29, 2002·Applied and Environmental Microbiology·Robert GelsominoJ Swings
Dec 27, 2002·The Journal of General and Applied Microbiology·Akira ManomeKazuo Komagata
Nov 17, 2004·International Journal of Systematic and Evolutionary Microbiology·Isao YumotoKenji Nakajima
Nov 24, 2004·Applied Microbiology and Biotechnology·M TaniguchiT Tanaka
Jan 18, 2005·International Journal of Systematic and Evolutionary Microbiology·Laurent ToffinDaniel Prieur
Jul 15, 2005·International Journal of Systematic and Evolutionary Microbiology·Kenji NakajimaIsao Yumoto
Nov 10, 2005·Biochimica Et Biophysica Acta·Etana PadanTerry A Krulwich
Feb 21, 2007·Letters in Applied Microbiology·M IshikawaK Yamasato
Apr 10, 2008·International Journal of Systematic and Evolutionary Microbiology·Isao YumotoKenji Nakajima
Jan 13, 2009·Journal of Bioscience and Bioengineering·Vichien KitpreechavanichKenji Sakai
Oct 30, 2009·International Journal of Molecular Sciences·Yutaka TokiwaSeiichi Aiba
Mar 4, 2011·Biotechnology Letters·Buenaventurada P CalabiaSeiichi Aiba

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