PMID: 3384010Apr 1, 1988Paper

Isolation of alpha-crystallin and its subunits by affinity chromatography on immobilized monoclonal antibodies

Experimental Eye Research
A Stevens, R C Augusteyn

Abstract

Use has been made of the specific interactions between monoclonal antibodies and the alpha A or alpha B subunits of alpha-crystallin to devise methods for the purification of the intact protein or its subunits. alpha A and alpha B subunits were separated by affinity chromatography on an immobilized monoclonal antibody specific for alpha A chains, using a pH gradient. Use of an antibody which binds both subunits has enabled the isolation of intact alpha-crystallin aggregates. Gel electrophoresis, conformational probing and size analysis showed that the affinity purified proteins were purer but otherwise indistinguishable from the alpha-crystallins isolated by other methods.

References

Aug 1, 1977·Experimental Eye Research·R J Truscott, R C Augusteyn
Sep 2, 1987·Biochimica Et Biophysica Acta·R C AugusteynJ A Thomson
Jan 1, 1985·Current Eye Research·R C AugusteynC H Thompson
Mar 1, 1985·Experimental Eye Research·J A Thomson, R C Augusteyn
Oct 1, 1985·The Australian Journal of Experimental Biology and Medical Science·J A ThomsonR C Augusteyn
Sep 28, 1971·Biochimica Et Biophysica Acta·J J GerdingH Bloemendal
Aug 12, 1969·Biochimica Et Biophysica Acta·H J HoendersH Bloemendal
Aug 1, 1983·European Journal of Biochemistry·N PuriR J Siezen
Oct 1, 1983·Experimental Eye Research·J A Thomson, R C Augusteyn
Jun 1, 1962·Experimental Eye Research·H BLOEMENDALJ H WISSE
Jul 2, 1962·Biochimica Et Biophysica Acta·J PAPACONSTANTINOUE SAITO

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Citations

Nov 23, 1988·Biochimica Et Biophysica Acta·R C AugusteynA Stevens
Feb 1, 1996·Current Eye Research·A StevensR C Augusteyn
Apr 6, 1992·Biochimica Et Biophysica Acta·K B MerckW W De Jong

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