Isolation of alpha-toxin, theta-toxin and kappa-toxin mutants of Clostridium perfringens by Tn916 mutagenesis

Microbial Pathogenesis
M M Awad, Julian I Rood

Abstract

Clostridium perfringens is the causative agent of clostridial myonecrosis or gas gangrene and mediates infection and disease by producing numerous extracellular toxins, including alpha-toxin, theta-toxin and kappa-toxin. Tn916-mutagenesis was used to isolate mutants defective in their ability to produce either alpha-toxin or theta-toxin. Nine independently derived mutants were isolated. In four of these mutants Tn916 had inserted at sites located 193 bp or 198 bp upstream of the theta-toxin structural gene, pfoA. Four mutants contained large deletions, three in regions which encompassed the theta-toxin structural and regulatory genes pfoA and pfoR, respectively, and the kappa-toxin structural gene, colA, and one in a region encompassing the alpha-toxin structural gene, plc. These mutants should prove to be invaluable for further genetic studies aimed at determining the role of these toxins in virulence.

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Citations

Mar 13, 2002·Journal of Bacteriology·Milena M Awad, Julian I Rood
Jan 19, 1999·Annual Review of Microbiology·J I Rood
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·E A Johnson, M Bradshaw
Feb 28, 2018·Frontiers in Microbiology·Rochelle C JosephNicholas R Sandoval
Jun 8, 2019·Microbiology Spectrum·S A KuehneD Lyras
Jul 28, 2019·Microbiology Spectrum·Masahiro NagahamaJulian I Rood

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