PMID: 8985429Jan 1, 1997Paper

Isolation of an amino-terminal region of bovine papillomavirus type 1 E1 protein that retains origin binding and E2 interaction capacity

Journal of Virology
X LengV G Wilson

Abstract

In vitro DNA binding results from a series of E1 proteins containing amino-terminal or carboxy-terminal truncations indicated that sequences between amino acids 121 and 284 were critical for origin binding. Additional binding experiments with E1 proteins containing internal, in-frame insertions or deletions confirmed the importance of the region defined by truncated E1 proteins and also demonstrated that downstream sequences were not required for binding activity in the context of the full-length E1 protein. On the basis of mapping results from the E1 mutants, a clone (pE1(121-311)) was constructed that expressed E1 amino acids within the approximate boundaries of the critical sequences for DNA binding. The E1(121-311) protein retained origin-specific DNA binding, confirming that this region was not only necessary but was also sufficient for origin recognition. In addition to origin binding, E1(121-311) bound E2 protein in a cold-sensitive manner. Therefore, DNA binding and E2 binding activities colocalize to a 191-amino-acid functional domain derived from the amino-terminal half of the E1 protein. Finally, three E1 proteins with mutations in this region all lacked DNA binding activity and were all defective for in vivo replica...Continue Reading

References

Jan 6, 1992·Biochimica Et Biophysica Acta·V G Wilson, J Ludes-Meyers
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M Lusky, E Fontane
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·P ParkI J Mohr
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·T G GilletteJ A Borowiec
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Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L YangM Botchan

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Citations

Jul 19, 2000·The Journal of General Virology·G D McShan, V G Wilson
May 3, 2013·Journal of Virology·Stephen SchuckArne Stenlund
Jul 16, 2013·Virology·Alison A McBride

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