Isolation of an antifungal Paenibacillus strain HT16 from locusts and purification of its medium-dependent antagonistic component

Journal of Applied Microbiology
W-W ZhouT-G Niu

Abstract

To isolate an antagonist for use in the biological control of the phytopathogenic fungus Penicillium expansum and purify the antifungal component produced by the antagonist. An antifungal strain HT16 was isolated from locusts, showing strong inhibition to Pen. expansum. Based on its in vitro effectiveness, HT16 was characterized as a strain of Paenibacillus polymyxa by phenotypic tests and 16S rDNA sequence analysis. It was found that the antifungal component HT16 secreted was only induced by Poria cocos sclerotium (PCS), and it remained active after sterilization at 121 degrees C for 15 min. The protein was purified by ammonium sulfate precipitation, heating process, and ultrafiltration using a 10 kDa cut-off membrane. The molecular weight of the purified antifungal protein, which was determined by mass spectrometry, was 4517 Da. A novel bacterial strain HT16 antagonistic to Pen. expansum was isolated from locusts and identified as Pae. polymyxa. The antifungal protein of 4517 Da was purified, and its production needed the inducer PCS in the fermentation medium. The antagonistic protein from Pae. polymyxa showed strong antifungal activity against phytopathogenic fungus Pen. expansum. This strain HT16 and the antifungal metabol...Continue Reading

References

Jul 1, 1984·Journal of General Microbiology·N A Logan, R C Berkeley
Jul 1, 1994·The Journal of Applied Bacteriology·M LebbadiE Valdivia
Nov 15, 1995·FEMS Microbiology Letters·B PichardD Thouvenot
May 13, 1999·International Journal of Systematic Bacteriology·Y R ChungK S Bae
Aug 7, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·P HelistöT Korpela
Sep 29, 2001·Journal of Applied Microbiology·S G Edwards, B Seddon
Nov 1, 1961·Archives of Biochemistry and Biophysics·P J VAN VUURENW J SERFONTEIN
Mar 5, 2004·International Journal of Food Microbiology·Davide Spadaro, Maria Lodovica Gullino
Mar 30, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yue-Yawn Chen, Hung-Min Chang
Oct 14, 2004·Journal of Applied Microbiology·P I KimY-T Chi
Mar 1, 1990·Applied and Environmental Microbiology·J HandelsmanC R Grau

❮ Previous
Next ❯

Citations

Aug 17, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Mileidy Cruz-MartínYelenys Alvarado-Capó

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.