PMID: 7017392May 1, 1981Paper

Isolation of an azide mutagenic metabolite in Salmonella typhimurium

Mutation Research
W M OwaisR A Nilan

Abstract

A scheme that employs a cation-exchange column and high-pressure liquid chromatography (HPLC) is devised to isolate and process large quantities of azide metabolite produced by S. typhimurium TA1530 strain. The mutagenic metabolite adheres strongly to the cation-exchange column, thus providing a convenient way to separate the metabolite from unreacted azide (N3-). The metabolite is very polar and only sparingly soluble in most organic solvents. Recrystallization in a methanol-carbon tetrachloride solvent system gave rise to microcrystalline material that decomposes with charring and gas evolution at 173-176 degrees C. The infrared spectrum indicates the presence of a covalently bound azide moiety.

References

Apr 1, 1978·Analytical Biochemistry·A A White, D B Karr
Jan 1, 1978·Mutation Research·A KleinhofsR A Nilan
Jul 1, 1979·Mutation Research·S de FloraC Bennicelli

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Citations

Feb 1, 1988·Mutation Research·W M Owais, A Kleinhofs
Mar 17, 1997·Mutation Research·E González-César, P Ramos-Morales
Mar 1, 1980·Mutation Research·J A JonesA Kleinhofs
Apr 1, 1988·Biochemical Medicine and Metabolic Biology·A A HunaitiW M Owais
Jan 1, 1989·Environmental and Molecular Mutagenesis·P Arenaz, L Hallberg

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