PMID: 2492093Jan 11, 1989Paper

Isolation of an IgH gene circular DNA clone from human bone marrow

Nucleic Acids Research
M Abe, H Shiku

Abstract

Circular DNA was obtained from human bone marrow. Then a phage library was prepared and screened by use of two probes of the IgH gene; 5'-DHQ52, containing the 5' flanking region of DHQ52, and JH4.3, containing the sequence from JH3 to the 3' flanking region of JH6. One clone, HBMC-1, that was DHQ52+JH4.3- was obtained. HBMC-1 had the germline IgH region upstream of JH1 and the 3' flanking region of DXP1. A recombination signal sequence flanking the 5' side of the JH1 segment was attached to the recombination signal sequence flanking the 3' side of DXP1 forming a head-to-head structure of two 7mers with 10 nucleotides in-between. HBMC-1 is thus considered to be a circular DNA deleted as a consequence of DXP1-JH1 joining of the IgH gene.

Citations

Jul 13, 2002·The Journal of Pathology·Mitsuyoshi HashimotoNaoyoshi Mori
Mar 1, 1997·Immunotechnology : an International Journal of Immunological Engineering·S FiorentiniB A Watkins

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