PMID: 6403945Apr 1, 1983Paper

Isolation of an intact DNA polymerase-primase from embryos of Drosophila melanogaster

Proceedings of the National Academy of Sciences of the United States of America
L S KaguniI R Lehman

Abstract

A procedure has been devised for the purification of intact DNA polymerase alpha from early embryos of Drosophila melanogaster. The purified enzyme consists of at least three polypeptides with Mrs of 182,000, 60,000, and 50,000. These are related antigenically to the alpha (Mr 148,000), beta (Mr 58,000), and gamma (Mr 46,000) subunits, respectively, of the DNA polymerase described previously [Banks, G. R., Boezi, J. A. & Lehman, I. R. (1979) J. Biol. Chem. 254, 9886-9892]. The alpha subunit (Mr 182,000) has a molecular weight indistinguishable from that observed in extracts of freshly harvested embryos and presumably present in vivo. As in the previous preparation, the alpha subunit is required for DNA polymerase activity and is very likely the catalytic subunit of the enzyme. The ratio of primase to polymerase remains constant throughout the purification. Thus, the primase is very likely an integral component of the Drosophila DNA polymerase alpha. The purified DNA polymerase-primase contains no detectable endo- or exodeoxyribonuclease and has pH, MgCl2, (NH4)2SO4, and NaCl optima identical to those reported previously. In contrast, the Km for dTTP is 3.7 microM as compared with 17.5 microM for the previous enzyme. Sensitiviti...Continue Reading

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Citations

Jul 1, 1984·Plant Molecular Biology·J GravelineS Litvak
Sep 8, 2011·European Biophysics Journal : EBJ·Ping Xie
Jul 1, 1988·Mutation Research·M F Goodman
Jul 13, 1988·Biochimica Et Biophysica Acta·L S Kaguni, I R Lehman
Aug 21, 2007·Nature Structural & Molecular Biology·Sebastian KlingeLuca Pellegrini
May 20, 2003·European Journal of Biochemistry·Satoshi NamekawaKengo Sakaguchi
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E KarawyaS H Wilson
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S M CotterillI R Lehman
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J PetruskaI Tinoco
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C S ChiangI R Lehman
Aug 25, 1988·Nucleic Acids Research·M A MillerT S Wang
Nov 11, 1992·Nucleic Acids Research·V M PeckA E Cress
Jun 15, 1987·European Journal of Biochemistry·Y F Roth
Oct 15, 1991·European Journal of Biochemistry·S Niranjanakumari, K P Gopinathan
May 15, 1992·European Journal of Biochemistry·V N PodustO I Lavrik
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May 15, 1984·Journal of Molecular Biology·R T HayM L DePamphilis
Oct 15, 1985·Biochemical and Biophysical Research Communications·F HiroseA Matsukage
Feb 24, 2015·DNA Repair·Roxana GeorgescuMike O'Donnell
Dec 15, 1987·Biochemistry·H P Nasheuer, F Grosse
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·A G So, K M Downey
Feb 1, 1993·FEBS Letters·S K Lee, M S Fuchs

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