PMID: 9182872Jan 1, 1997Paper

Isolation of anti-mesothelin antibodies from a phage display library

Molecular Immunology
P ChowdhuryI Pastan

Abstract

Phage display has been used to select single-chain Fvs (scFvs) against mesothelin, a surface antigen present on mesothelial cells as well as mesotheliomas and non-mucinous ovarian cancers. Several attempts to produce anti-mesothelin hybridomas from spleen cells of mice immunized with recombinant mesothelin were unsuccessful. This report describes the isolation of anti-mesothelin scFvs from a phage display library made from the mRNA of the same spleens. Panning on recombinant antigen produced in E. coli or on antigen positive cells was employed. Several scFvs which bind specifically to mesothelin were isolated. Panning on recombinant antigen yielded five different scFvs. Panning on cells selected two different scFvs which also differ from the scFvs selected by recombinant antigen. The heavy chains of the scFvs selected on recombinant antigen are derived from four different heavy chain gene families and the scFvs selected on cells are derived from two of these families. In contrast, the light chains of all of these scFvs are derived from family XI. Moreover, the light chains of the two scFvs selected on cells are very similar to the light chains of two of the scFvs selected by panning on recombinant mesothelin except for a few po...Continue Reading

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Citations

Feb 28, 2001·Journal of Immunological Methods·P S ChowdhuryI Pastan
May 7, 1999·Immunotechnology : an International Journal of Immunological Engineering·B JwangI Pastan
Feb 26, 2000·European Journal of Biochemistry·L K JespersenJ Engberg
Aug 13, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Robert J KreitmanIra Pastan
Oct 10, 2006·The AAPS Journal·Robert J Kreitman
Aug 18, 2009·Biomarkers in Medicine·Dimitra SasaroliNathalie Scholler
Feb 13, 2016·Immunological Reviews·Ronit MazorIra Pastan
Mar 5, 2016·Expert Review of Anticancer Therapy·Laurel M SchunselaarPaul Baas
Nov 18, 2014·Biochemical Society Transactions·Andrew C R Martin
Jul 5, 2005·Expert Opinion on Investigational Drugs·R J Kreitman
Jan 16, 2002·Journal of Receptor and Signal Transduction Research·M Dani

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