PMID: 3750345Jan 1, 1986Paper

Isolation of antihemorrhagic factors in opossum (Didelphis virginiana) serum using a monoclonal antibody immunoadsorbent

Toxicon : Official Journal of the International Society on Toxinology
S F TarngJ C Pérez

Abstract

The antihemorrhagic factor in opossum (Didelphis virginiana) serum isolated by Sephadex G-200 gel filtration and DEAE A-50 ion exchange chromatography was used as antigen to immunize BALB/c mice. Hybrid cell lines secreting monoclonal antibodies against antihemorrhagic factor were produced by fusion of Sp2/0 myeloma cells with spleen cells of the immunized mice. The ascites fluid was produced in BALB/c mice. The monoclonal antibody in the ascites fluid was partially purified by DEAE A-50 ion exchange and coupled to CNBr-activated isolation of isolation of antihemorrhagic factor. The neutralization capacity of the conventionally isolated antihemorrhagic factor was 14.6 times and the affinity isolated antihemorrhagic factor was 16.8 times that of crude opossum serum. Both antihemorrhagic factors were homogeneous, with one fast migrating band in the area of albumin shown by polyacrylamide gel electrophoresis. However, the antihemorrhagic factor showed one heavy band and one faint band in SDS-polyacrylamide electrophoresis, as well as in isoelectric focusing. The molecular weight of the heavy band was estimated to be 65,000 with a value of p1 4.8 and the faint band was 57,000 with a value of pI 4.1.

References

Jan 1, 1978·Toxicon : Official Journal of the International Society on Toxinology·J C PerezC H Hatch
Jan 1, 1978·Toxicon : Official Journal of the International Society on Toxinology·J C PerezB M Jennings
Jan 1, 1978·Toxicon : Official Journal of the International Society on Toxinology·M Ovadia
Feb 1, 1976·Journal of Clinical Pathology·A VollerB Oladehin
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·T A EgorovJ Carlsson
May 27, 1983·Journal of Immunological Methods·M HeikinheimoH Bohn
Jan 1, 1984·Toxicon : Official Journal of the International Society on Toxinology·V E Garcia, J C Perez
Jan 1, 1983·Hybridoma·S WeissM Cohn
Jan 1, 1984·Toxicon : Official Journal of the International Society on Toxinology·J C PerezS Y Huang
Jan 1, 1981·Toxicon : Official Journal of the International Society on Toxinology·J M Menchaca, J C Perez

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Citations

Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·C L Fortes-DiasC R Diniz
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·G B DomontH Moussatché
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·S WeissenbergE Kochva
Oct 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·T Omori-SatohD Mebs
Dec 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·Morgan R McKeller, John C Pérez
Jul 24, 2003·Toxicon : Official Journal of the International Society on Toxinology·Lawan ChanhomeVisith Sitprija
Jun 10, 1998·Toxicon : Official Journal of the International Society on Toxinology·T Omori-SatohD Mebs
Jun 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·G BorkowM Ovadia
Apr 29, 1999·Toxicon : Official Journal of the International Society on Toxinology·J C Pérez, E E Sánchez
May 26, 1999·Toxicon : Official Journal of the International Society on Toxinology·R R MartinezR Campos
Mar 23, 2015·Toxicon : Official Journal of the International Society on Toxinology·Danielle H DrabeckSharon A Jansa
Aug 8, 2008·Immunopharmacology and Immunotoxicology·Elda E Sánchez, Alexis Rodríguez-Acosta

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