Isolation of antiSLIP1-reactive boar sperm P68/62 and its binding to mammalian zona pellucida

Molecular Reproduction and Development
N TanphaichitrD White

Abstract

Single-step purification of boar sperm P68/62 that is cross-reactive with a polyclonal antibody against sulfolipidimmobilizing protein 1 (SLIP1) was achieved by chromatofocusing. This method is useful for obtaining P68/62 in quantity. The two proteins, P68 and P62, were antigenically related, since the antibody generated specifically against the 68-kDa band reacted with both the 68- and 62-kDa bands. Like rat testis SLIP1, purified boar sperm P68/62 bound to sulfogalactosylglycerolipid (SGG) and inhibited sperm-egg binding in a dose-dependent manner when added exogenously to sperm-egg coincubates. This inhibitory effect occurred at the level of the zona pellucida (ZP), and further studies showed that biotinylated boar sperm P68/62 bound to the ZP of unfertilized mouse eggs. Furthermore, biotinylated boar sperm P68/62 bound to isolated ZP of unfertilized eggs from other species, including pig, rat, cat, dog, and human, as well as to ZP of intact fertilized mouse eggs and preimplantation embryos of various developmental stages, although the degree of its binding to the ZP of intact eight-cell embryos, morulae, and blastocysts was much lower than that of fertilized eggs and two-cell embryos. These results suggest that P68/62 of ca...Continue Reading

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Citations

Nov 7, 2000·Biochimica Et Biophysica Acta·F M Flesch, B M Gadella
Jun 21, 2002·Developmental Biology·Euridice CarmonaNongnuj Tanphaichitr
Aug 24, 1999·Biology of Reproduction·V AhnonkitpanitN Tanphaichitr
May 22, 2002·Biology of Reproduction·Euridice CarmonaNongnuj Tanphaichitr
Jun 22, 2000·Biology of Reproduction·D WhiteN Tanphaichitr
Jun 25, 2002·Biology of Reproduction·Julierut TantibhedhyangkulNongnuj Tanphaichitr
Jun 23, 2001·Molecular Human Reproduction·M RattanachaiyanontN Tanphaichitr
Mar 7, 2020·Cell and Tissue Research·Michal ZigoPeter Sutovsky

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