Isolation of bacteriophages from the oral cavity

Letters in Applied Microbiology
G HitchP W Taylor

Abstract

To isolate bacteriophages lytic for oral pathogens from human saliva, dental plaque and mature biofilms constituted from saliva-derived bacteria. Saliva and dental plaque samples from healthy volunteers and from patients with gingivitis and periodontitis were examined for the presence of lytic bacteriophage using a panel of oral pathogens and bacteria isolated from the samples. Samples were also enriched for bacteriophage using static culture techniques and mature biofilms. A limited number of samples contained bacteriophage particles that were visualized using electron microscopy. Cultures yielded phage infecting non-oral bacteria (Proteus mirabilis) but no bacteriophage specific for recognized oral pathogens were found. Some micro-organisms from the oral microflora elaborated antibacterial substances that inhibited growth of other residents of the oral cavity. Unlike other ecosystems, the composition of the oral cavity does not appear to be heavily influenced by interactions between bacteriophages and their hosts. Bacteriophage for control of oral infections may need to be obtained from other sources. Antibacterial substances derived from some members of the oral microflora warrant investigation as potential antibiotics.

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Citations

Jan 31, 2012·Applied and Environmental Microbiology·Allan L DelisleSylvain Moineau
Apr 9, 2013·Journal of Oral Microbiology·Carla CuginiMary E Davey
May 30, 2014·The Journal of Antimicrobial Chemotherapy·Tania Mareike ViertelHans-Peter Horz
Jan 14, 2016·Virus Genes·Yogesh A KarpeKalyan Banerjee
Oct 18, 2008·Current Opinion in Biotechnology·Michael Mattey, Janice Spencer
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Aug 26, 2006·Periodontology 2000·Adam P Roberts, Peter Mullany
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Jan 8, 2015·Frontiers in Microbiology·Vinh T Dang, Matthew B Sullivan
Aug 23, 2007·FEMS Microbiology Letters·Jan R van der Ploeg
Jun 24, 2016·PLoS Pathogens·Adam WahidaHans-Peter Horz
Jan 22, 2017·Journal of Biotechnology·Szymon P SzafrańskiMeike Stiesch
Jan 8, 2017·Applied and Environmental Microbiology·Wen-Chun ChouYi-Ywan M Chen
Jul 28, 2017·Journal of Dental Research, Dental Clinics, Dental Prospects·Manisha Chandwani, Shweta Chandak
Dec 8, 2010·Expert Review of Anti-infective Therapy·Adam P Roberts, Peter Mullany
Jan 29, 2014·Journal of Basic and Clinical Physiology and Pharmacology·Negin KianiBijan Heidari
Sep 10, 2020·Cellular & Molecular Immunology·Sara FedericiEran Elinav
Aug 26, 2017·Microbiology Spectrum·Anilei HoarePatricia I Diaz
Jul 2, 2014·BMC Microbiology·Mayuri NaiduDavid T Pride

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